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Site-Specific Incorporation of Sulfotyrosine into Proteins in Mammalian Cells
Protein tyrosine O-sulfationposttranslational modifications (PTMs)tyrosine O-sulfation (PTS) plays a crucial role in numerous extracellular...
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Mammalian Semiochemicals
Progress that has been made in research on the chemical aspects of mammalian semiochemistry over the past decade is discussed on the basis of...
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Anti-CRISPR Proteins and Their Application to Control CRISPR Effectors in Mammalian Systems
CRISPR-Cas effectors are powerful tools for genome and transcriptome targeting and editing. Naturally, these protein–RNA complexes are part of the...
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Engineered Protease-Responsive RNA-Binding Proteins (RBPs) to Expand the Toolbox of Synthetic Circuits in Mammalian Cells
Genetically encoded sensor–actuator circuits aim at reprogramming cellular functions and are inspired by intracellular networks: from the input...
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Polycomb Group Proteins Methods and Protocols
This volume explores the latest technologies used in the Polycomb Group of proteins field and helps scientists--working on PcG proteins--investigate...
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Recombinant Protein Expression in Mammalian Cells Methods and Protocols
This fully updated volume explores notable developments in the field of mammalian cell-based recombinant protein production. Beginning with methods...
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Purification and Quality Control of Recombinant Proteins Expressed in Mammalian Cells: A Practical Review
In the recent years, there has been a rapid development of new technologies and strategies when it comes to protein purification and quality control...
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Osmium-Resistant Fluorescent Proteins and In-Resin Correlative Light-Electron Microscopy of Epon-Embedded Mammalian Cultured Cells
Postfixation with osmium tetroxide and Epon embedding are essential for the preservation and visualization of subcellular ultrastructures via...
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Coiled-Coil Interaction Toolbox for Engineering Mammalian Cells
Protein interactions play a crucial role in a variety of biological processes. Therefore, regulation of these interactions has received considerable...
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RNA Switches Using Cas Proteins
Expanding the number of available RNA-binding proteins (RBPs) is vital to establishing posttranscriptional circuits in mammalian cells. We focused on...
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Mammalian Antigen Display for Pandemic Countermeasures
Pandemic countermeasures require the rapid design of antigens for vaccines, profiling patient antibody responses, assessing antigen...
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Genetic Code Expansion in Mammalian Cells
The expansion of the genetic code has enabled the incorporation of noncanonical amino acids (ncAAs) into a defined site of proteins. By introducing...
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Single-Step Non-Chromatographic Purification of Recombinant Mammalian Proteins Using a Split Intein ELP Tag System
Glycoprotein therapeutics are currently used by large patient populations and generate significant revenue for the biopharmaceutical industry. These...
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Mammalian Cell Culture: An Edge to Biopharmaceutical Industry
The future of mammalian cell culture is bright; thus, it is expanding. Many challenges remain, and new opportunities will emerge. This chapter...
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Mechanistic Model-Driven Biodesign in Mammalian Synthetic Biology
Mathematical modeling plays a vital role in mammalian synthetic biology by providing a framework to design and optimize design circuits and...
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Flux Balance Analysis of Mammalian Cell Systems
Flux balance analysis (FBA) is a computational methodology to model and analyze the metabolic behavior of cells. In this chapter, we break down the...
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In Vitro Reconstitution Platforms of Mammalian Cell-Free Expressed Membrane Proteins
Membrane proteinsMembrane proteins are essential components in cell membranes and enable cells to communicate with their outside environment and to...
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Mammalian Fetal Organ Regeneration
The develo** fetus has the remarkable ability to heal dermal skin wounds by regenerating normal epidermis and dermis with restoration of the...
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Mammalian Synbio Sensors
Mammalian cells behave as computers: they operate as information-processing systems that dynamically integrate and respond to diverse environmental...
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The Use of Baculovirus-Mediated Gene Expression in Mammalian Cells for Recombinant Protein Production
Baculovirus-mediated gene expression in mammalian cells, BacMam, is a useful alternative to transient transfection for recombinant protein production...