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The reaction mechanism of the Ideonella sakaiensis PETase enzyme
Polyethylene terephthalate (PET), the most abundantly produced polyester plastic, can be depolymerized by the Ideonella sakaiensis PETase enzyme....
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An explainability framework for deep learning on chemical reactions exemplified by enzyme-catalysed reaction classification
Assigning or proposing a catalysing enzyme given a chemical or biochemical reaction is of great interest to life sciences and chemistry alike. The...
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Biosynthesis of Feruloyl Glycerol from Ferulic Acid and Glycerol Through a Two-Enzyme Cascade Reaction
Feruloyl glycerol (FG) has a variety of biological activities, but the green synthesis methods of FG remain rare. In this study, FG was prepared by a...
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Wool keratin-based colorimetric detection of organophosphorus pesticides via a multi-enzyme cascade reaction
A simple and sensitive colorimetric method for organophosphorus pesticides (Ops) detection was proposed via a multi-enzyme cascade reaction. The...
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Glucose Determination by Enzyme Analysis
Enzyme analysis is used for many purposes in food science and technology, including to indicate adequate processing, to assess enzyme preparations,...
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Enzyme-free nucleic acid dual-amplification strategy combined with mimic enzyme catalytic precipitation reaction for the photoelectrochemical detection of microRNA-21
A novel photoelectrochemical (PEC) biosensor based on an enzyme-free nucleic acid dual-amplification strategy combined with a mimic enzyme to...
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Improving efficiency and reducing enzyme inactivation during lipase-mediated epoxidation of α-pinene in a double-phase reaction system
Chemoenzymatic epoxidation of olefin mediated by lipase is a green and environmentally friendly alternative process. However, the mass transfer...
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Basic Concepts and Applications of Enzyme-Catalyzed Reactions for Biotechnology
Enzyme-catalyzed reactions provide several advantages such as specificity, high catalytic rate, transition state stabilization, etc., over inorganic...
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Bioconjugation of nanozyme and natural enzyme to enable a one-step cascade reaction for the detection of metabolites
Nanozyme, with enzyme-mimicking activity and excellent stability, has attracted extensive attention. However, some inherent disadvantages, including...
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In Vitro Enzyme-Induced Vinyl Polymerization
The in vitro enzyme-mediated polymerization of vinyl monomers is reviewed with a scope covering enzymatic polymerization of vitamin C...
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Modeling Enzyme-Substrate Interaction with Localization-Delocalization Matrices
Enzymes are life’s catalysts par excellence. They are incredibly efficient and are mainly composed of protein—save prosthetic groups and/or nucleic...
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Reaction Kinetics
This chapter deals with the rates of reactions commonly occurring in food systems, and considers chemical reactions, reactions occurring in...
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Full reaction mechanism of hydrogen peroxide catalyzed by reductive CoP nanoparticles: the enzyme-like activity
The hydrogen peroxide (H 2 O 2 ) catalyzed by nanoparticles (NPs) demonstrates potential broad applications in the field of biomedicine and environmental...
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Catalytic Mechanisms and Active Species of Benzene Hydroxylation Reaction System Based on Fe-Based Enzyme-Mimetic Structure
Subject to the shortcomings of traditional heterogeneous materials (such as the limited controllability of the preparation process, the complex...
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Reaction-diffusion hydrogels from urease enzyme particles for patterned coatings
The reaction and diffusion of small molecules is used to initiate the formation of protective polymeric layers, or biofilms, that attach cells to...
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Immobilization of lipase enzyme onto herbal silver nanoparticles and examination of biochemical parameters of immobilized enzyme
Nanoparticles are useful for immobilization due to their size and physical properties. The present study aimed to synthesize herbal silver...
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Redesigning Robust Biocatalysts by Engineering Enzyme Microenvironment and Enzyme Immobilization
Enzymes have replaced traditional industrial catalysts as more efficient, eco-friendly, and sustainable alternatives that can be used in different...
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Immobilized enzyme cascade for targeted glycosylation
Glycosylation is a critical post-translational protein modification that affects folding, half-life and functionality. Glycosylation is a...
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Engineering an efficient and enantioselective enzyme for the Morita–Baylis–Hillman reaction
The combination of computational design and directed evolution could offer a general strategy to create enzymes with new functions. So far, this...
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Enzyme-Assisted Extraction
The extraction of compounds from plant and animal sources that have medicinal properties are known as bioactive compounds, which can either improve...