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Enzyme-Linked Immunosorbent Assay-Based Nanosensors for the Detection of Pathogenic Bacteria
Since its inception, enzyme-linked immunosorbent assay (ELISA) has been one of the promising methods for detecting bacterial pathogens. Although the... -
Dual-probe ligation without PCR for fluorescent sandwich assay of EGFR nucleotide variants in magnetic gene capture platform
A simple, rapid, and highly efficient fluorescent detection technique without PCR through dual-probe ligation with the genetic capture of magnetic...
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A dual-emission ratiometric fluorescence strategy with enzyme-based inhibition for organophosphorus pesticides determination
A fast, eco-friendly and accurate ratiometric fluorescent strategy is presented for the determination of organophosphorus pesticides (OPs) using...
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A pH/enzyme dual responsive PMB spatiotemporal release hydrogel promoting chronic wound repair
Suppressing persistent multidrug-resistant (MDR) bacterial infections and excessive inflammation is the key for treating chronic wounds. Therefore,...
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Enzyme-free nucleic acid dual-amplification strategy combined with mimic enzyme catalytic precipitation reaction for the photoelectrochemical detection of microRNA-21
A novel photoelectrochemical (PEC) biosensor based on an enzyme-free nucleic acid dual-amplification strategy combined with a mimic enzyme to...
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A dual-mode biosensor for salivary cortisol with antibody-aptamer sandwich pattern and enzyme catalytic amplification
A split-type and dual-mode (photoelectrochemical and electrochemical, PEC and EC) biosensor was developed for non-invasive and sensitive detection of...
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Spectrally separated dual-label upconversion luminescence lateral flow assay for cancer-specific STn-glycosylation in CA125 and CA15-3
Multiplexed lateral flow assays (LFAs) offer efficient on-site testing by simultaneously detecting multiple biomarkers from a single sample, reducing...
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pH-responsive dual-enzyme mimics based on hollow metal organic framework-derivatives β-Co(OH)2 for multiple colorimetric assays
AbstractA hollow metal organic framework derivative β-Co(OH) 2 has been prepared, which possesses oxidase and peroxidase-like activities....
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Ratio-fluorescent and naked-eye visualized dual-channel sensing strategy for Cu2+ and alkaline phosphatase activity assay
The levels of Cu 2+ and alkaline phosphatase (ALP) are the important indicators of the developed stage of the relative diseases. Herein, a binary...
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Superior oxidase-mimetic activity of FeCo-NC dual-atom nanozyme for smartphone-based visually colorimetric assay of organophosphorus pesticides
A colorimetric analysis platform has been successfully developed based on FeCo-NC dual-atom nanozyme (FeCo-NC DAzyme) for the detection of...
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Design of Enhanced Smart Delivery Systems for Therapeutic Enzymes: Kinetic and Release Performance of Dual Effected Enzyme-Loaded Nanopolymers
L-asparaginase (L-ASNase) is among the important biopharmaceuticals and is utilized in the treatment of acute lymphoblastic. L-ASNase catalyzes its...
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Fabrication of a dual mimetic enzyme sensor based on gold nanoparticles modified with Cu(II)-coordinated methanobactin for gallic acid detection
Gallic acid (GA) is a bioactive functional ingredient widely used in pharmacology and food. For GA detection, the accuracy and selectivity can be...
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Utilizing dual carriers assisted by enzyme digestion chemiluminescence signal enhancement strategy simultaneously detect tumor markers CEA and AFP
To measure two tumor biomarkers, alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA), a dual-carrier CL sensor with restriction enzyme...
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Rapid qualitative and quantitative detection of Salmonella typhimurium using a single-step dual photometric/fluorometric assay
A dual-signal photometric/fluorometric assay was established for rapid, qualitative, and quantitative detection of Salmonella typhimurium ( S....
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Colorimetric sensing of ascorbic acid using the catalytic activity of β-Mn2V2O7 nanoparticles as dual enzyme-mimic
Enzyme-mimetic reactions have led to the development of rapid and cost-effective visual sensors for colorimetric detection of essential molecules. In...
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Dual-stimuli-responsive porous polymer enzyme reactor for tuning enzymolysis efficiency
A strategy for preparing a dual-stimuli-responsive porous polymer membrane enzyme reactor (D-PPMER) is described, consisting of poly (styrene-maleic...
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DNA tetrahedron-based dual-signal fluorescence detection of apoE4 gene sites on a microplate reader
As a neurodegenerative disorder, Alzheimer’s disease (AD) is characterized by cognitive dysfunction and behavioral impairment. Among the various...
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An enzymatic nucleic acid vertical flow assay
Vertical flow assays have been developed in recent years addressing limitations of the lateral flow assays, including limited multiplexing...
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One-Pot Biosynthesis of 3-Aminopropionic Acid from Fumaric Acid Using Recombinant Bacillus megaterium Containing a Linear Dual-Enzyme Cascade
3-Aminopropionic acid (3-APA) has wide applications in food, cosmetics, pharmaceuticals, chemical, and polymer industries. This present study aimed...
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Green synthesis of zinc and nickel dual-doped cerium oxide nanoparticles: antioxidant activity and cytotoxicity effects
Cerium oxide nanoparticles (CeO 2 -NPs) and Zn–Ni dual-doped CeO 2 -NPs were synthesized through a green approach by the implication of zucchini peel ( Cuc...