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Measuring Antibody-Mediated Tau Fibril Uptake in Microglia by Flow Cytometry
Microglia are brain-resident phagocytic cells, considered to be the innate immune cells of the central nervous system. Microglia respond to both...
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Degradation of Chlorpyrifos Pesticide Using a Postbiotic Enzyme of Lactic Acid Bacteria
Lactic acid bacteria (LAB) are well known for their production of postbiotic components such as organic acids, short-chain fatty acids, enzymes,...
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A Mass Spectrometry-Based Strategy for Map** Modification Sites for the Ubiquitin-Like Modifier NEDD8
The identification of modification sites for ubiquitin and ubiquitin-like modifiers is an essential step in the elucidation of controlled processes....
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Generation of Red Blood Cell Nanovesicles as a Delivery Tool
Extracellular vesicles (EVs) are natural membranous vesicles with immense potential as drug delivery tools. However, their large-scale production...
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Update DESI Mass Spectrometry Imaging (MSI)
Desorption electrospray ionization (DESI) is an ambient technique that allows chemical information to be obtained directly from a wide range of...
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Calcium-Phosphate X-ray Contrast Cements for Bone Repair
AbstractCalcium phosphate composite cements for bone grafting containing an X-ray contrast agent, (BiO) 2 CO 3 , were obtained. Its presence in cement...
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Methods to Measure NLR Oligomerization I: Size Exclusion Chromatography, Co-immunoprecipitation, and Cross-Linking
Protein oligomerization is a common principle of regulating cellular responses. Oligomerization of NLRs is essential for the formation of NLR...
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Manufacturing the Biopharmaceutical Drug Product
Manufacture of the finished drug product is the last process step (from starting materials to drug substance and now to drug product) for the...
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Characterizing Bioinks for Extrusion Bioprinting: Printability and Rheology
In recent years, new technologies based on 3D bioprinting have emerged as ideal tools with which to arrange cells and biomaterials in three...
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A Generalized Method for Metal Fixation in Horse Spleen L-Ferritin Cage
The naturally occurring iron storage protein, ferritin, has been recognized as an important template for preparing inorganic nanomaterials by...
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Constructing Nucleic Acid Delivering Lipoproteoplexes from Coiled-Coil Supercharged Protein and Cationic Liposomes
The safe and efficient delivery of nucleic acids is crucial for both clinical applications of gene therapy and pre-clinical laboratory research. Such...
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Development of an ultrasonic and heat-assisted liquid–liquid extraction method combined with deep eutectic solvent-based dispersive liquid–liquid microextraction for the extraction of some phytosterols from cow milk butter samples
In this study, an ultrasonic and heat-assisted liquid–liquid extraction method combined with deep eutectic solvent-based dispersive liquid–liquid...
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Food Microbiology and the Application of Nanotechnology in Food Processing and Safety
The engineering of functional units of tiny particles of nanoscale size is known as nanotechnology. It is a branch of science and technology that...
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Fully Integrated Online Strategy for Highly Sensitive Proteome Profiling
Low-input proteomics, which treats tens to hundreds of mammalian cells, is the gap between standard proteomics and single-cell proteomics. Low-input...
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Injectable Hydrogel for Drug Delivery
Injectable hydrogels are of gel technology in the laboratory that improves the need for gel kinetic control. They have a carrier property in three...
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Improved Coverage of the N-Terminome by Combining ChaFRADIC with Alternative Proteases
Many proteolytic cleavage events cannot be covered with conventional trypsin-based N-terminomics workflows. These typically involve the...
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Dietary Supplementation and Immunomodulatory Activity of Postbiotics in Fish
One of the most significant and promising segments of the global food system is aquaculture production. A key source of nutrition for people is fish....
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Analysis of Cholesterol from the Liver Using Gas Chromatography–Mass Spectrometry
Cholesterol is an essential lipid molecule for several biological functions including the proper functioning of cell membranes, lipoproteins, and...
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Determination of 2-Monochloropropane-1,3-Diol Esters (2-MCPDE), 3-Monochloropropane-1,2-Diol Esters (3-MCPDE) and Glycidyl Esters (GE) in Marine Oil-Based Supplement by Acid Transesterification Via Gas Chromatography Coupled to Mass Spectrometry (GC-MS)
Fish and algae oil can be commercialized as food supplement capsules and consumed daily due to their health-beneficial compounds as fatty acids and...
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Aquametry
The knowledge of the moisture in drugs/analytical samples is usually required to maintain the standards and for analytical reasons. Many...