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Glutamine Maintains Satellite Glial Cells Growth and Survival in Culture
Satellite glial cells (SGCs) tightly surround neurons and modulate sensory transmission in dorsal root ganglion (DRG). At present, the biological...
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Generation of Motor Neurons from Human Induced Pluripotent Stem Cells Using Small Chemical Molecules
Motor neurons (MNs) play a critical role in regulating both voluntary and involuntary movements, and their dysfunction underlies various...
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Integrated Approach for the Differentiation of Neurons and Astrocytes from Human Pluripotent Stem Cells
The differentiation of human pluripotent stem cells (hPSCs) into neural lineage cells is an important approach for studying neural induction, cell...
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Generation of Functional Microglia from Human Pluripotent Stem Cells
Microglia are resident macrophages that are unique in the central nervous system (CNS), including the brain, spinal cord, and retina. Microglia play...
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Preparation of Highly Pure hiPSC-Derived Motor Neurons Through Assembling a Co-culture System
Modeling motor neuron (MN)-related diseases using human induced pluripotent stem cells (hiPSCs) requires culture conditions replicating MN...
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Generation and Characterization of Human Induced Pluripotent Stem Cells via Retroviral Delivery of Yamanaka Factors
Limited access to human samples, especially those from patients with certain genetic defects, was a major challenge for studying disease pathogenesis...
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Lentiviral Delivery of Transcription Factors to Generate Motor Neurons from Human Induced Pluripotent Stem Cells
This protocol addresses the need for a reliable and scalable method for generating motor neurons (MNs) in vitro from human induced pluripotent stem...
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Directed Differentiation of Neurons from Human iPSCs for Modeling Neurological Disorders
Human-induced pluripotent stem cell (hiPSC) technology has enabled comprehensive human cell-based disease modeling in vitro. Due to limited...
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Preparation of Healthy Single Neuron or Astrocyte Suspension from Adult Mouse Brain for RNA-seq
Single-cell RNA sequencing (scRNA-seq) has been widely applied in neuroscience research, enabling the investigation of cellular heterogeneity at the...
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Generation of Homogeneous Populations of Cortical Interneurons from Human Pluripotent Stem Cells
Cortical interneurons (cINs), especially those that are derived from the medial ganglionic eminence (MGE) during early development, are associated...
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Generation of Human Induced Pluripotent Stem Cells Using Episomal Vectors
Human induced pluripotent stem cells (iPSCs) can be reprogrammed from differentiated somatic cells, such as adult fibroblasts and peripheral blood...
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Gene Editing and Reprogramming of Human Fibroblast Cells to Human Induced Pluripotent Stem Cells
Predictive disease models play significant roles in advancing our knowledge of the pathology of human disease. In this field, animal models have been...
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Serum-Free Production of Human Stem Cell-Derived Liver Spheres for Cancer Metastasis Research
Renewable and scalable human liver tissue platforms are a powerful tool to study organ physiology and model diseases, such as cancer. Stem...
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An Optimized Protocol of Electroporation of Hepatocyte Nuclear Factor 1 Alpha (Hnf-1α) in Mesenchymal Stem Cells
AbstractThe objective of this study was to investigate the optimization of electroporation of hepatocyte nuclear factor 1 alpha ( Hnf-1 α) in murine...
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Measuring Protein Nuclear Transport via a Dual Reporter in iPSC-Derived Neurons
Nucleocytoplasmic transport (NCT) plays a critical role in maintaining cellular homeostasis, and its dysregulation has been shown to be implicated in...
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Methods to Study the Role of Mechanical Signals in the Induction of Cancer Stem Cells
Microenvironmental mechanical signals are fundamental regulators of cell behavior both in physiological and in pathological context, particularly in...
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Generating Homogeneous Brain Organoids from Human iPSCs
There is a high demand for the development of in vitro models for human brain development and diseases due to the inaccessibility of human brain...
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Validation of Pluripotency of Human Induced Pluripotent Stem Cells
The pluripotency of human induced pluripotent stem cells (hiPSCs) is critical for the generation of different tissues and cell types in downstream...
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Real-Time Monitoring of RAS Activity Using In Vitro and In-Cell NMR Spectroscopy
The activation level of RAS can be determined by GTP hydrolysis rate (khy) and GDP-GTP exchange rates (kex). Either impaired GTP hydrolysis or...
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Differentiation of Human Embryonic/Induced-Pluripotent Stem Cells to Retinal Ganglion Cells
The generation of retinal ganglion cells (RGCs) differentiated from human embryonic stem cell (hESC) or induced-pluripotent stem cells (iPSC) could...