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Retinal Ganglion Cells

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Ben Mead

    1. School of Optometry and Vision Sciences, Cardiff University, Cardiff, UK

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2708)

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About this book

This collection provides methods and techniques employed when culturing, manipulating, quantifying, and functionally assessing retinal ganglion cells (RGCs), vital for better understanding various ocular diseases and for develo** novel therapies. The book features exhaustive and detailed protocols for the study of RGCs at both the in vitro and in vivo level, including the culture of rodent and human cells, and immunohistochemical, morphological, and functional assessments of various in vivo models of RGC death. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Retinal Ganglion Cells: Methods and Protocols serves as an ideal guide to these important cellularfeatures of the central nervous system and the many traumatic and degenerative blinding diseases that affect them.

Keywords

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xii
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  2. Isolation and Culture of Primary Retinal Ganglion Cells from Rodent Retina

    • Esmahan Durmaz, Matyas Kutnyanszky, Ben Mead
    Pages 1-10

  3. Purification of Retinal Ganglion Cells from Differentiation Through Adult via Immunopanning and Low-Pressure Flow Cytometry

    • Sean M. Riordan, Afnan M. Aladdad, Kiran J. McLoughlin, Karl E. Kador
    Pages 11-24

  4. Preparation of Retinal Explant Cultures

    • Zubair Ahmed
    Pages 25-31

  5. Gene Gun DiOlistic Labelling of Retinal Ganglion Cells

    • Gloria Cimaglia, Ryan J. Bevan, Andrew Want, James E. Morgan
    Pages 33-40

  6. Differentiation of Human Embryonic/Induced-Pluripotent Stem Cells to Retinal Ganglion Cells

    • Maryam Esmaeili, Ben Mead
    Pages 41-48

  7. Laser-Induced Ocular Hypertension in a Mouse Model of Glaucoma

    • José M. Ramírez, Elena Salobrar-García, Rosa de Hoz, Juan J. Salazar, José A. Matamoros, Lidia Sánchez-Puebla et al.
    Pages 49-56

  8. Silicone Oil-Induced Ocular Hypertension Glaucoma Model (SOHU) in Rodent and Nonhuman Primate

    • Fang Fang, Jie Zhang, Yang Hu
    Pages 57-69

  9. Microbead-Induced Ocular Hypertension in a Rodent Model of Glaucoma

    • Ben Mead
    Pages 71-76

  10. Viral Vector-Induced Ocular Hypertension in Mice

    • J. Cameron Millar, Yogapriya Sundaresan, Gulab S. Zode, Abbot F. Clark
    Pages 77-97

  11. Optic Nerve Crush Injury in Rodents to Study Retinal Ganglion Cell Neuroprotection and Regeneration

    • Zubair Ahmed
    Pages 99-106

  12. Optical Coherence Tomography: Imaging Visual System Structures in Mice

    • **angxiang Liu, Yuan Liu, Richard K. Lee
    Pages 107-113

  13. Confocal Scanning Laser Ophthalmoscopy to Image Retinal Ganglion Cells in Real-Time

    • Lili Hao, Yuan Liu, **angxiang Liu, Richard K. Lee
    Pages 115-121

  14. In Vivo Detection of Retinal Ganglion Cell Stress in Rodents with DARC

    • Daniel Hill, Soyoung Choi, Maria Francesca Cordeiro
    Pages 123-129

  15. Measuring the Full-Field Electroretinogram in Rodents

    • Pei Ying Lee, Da Zhao, Vickie H. Y. Wong, Anh Hoang, Katie K. N. Tran, Anna K. van Koeverden et al.
    Pages 131-140

  16. Evaluation of Retinal Ganglion Cell via Visual Evoked Potential

    • Shengjian Lu, Tian **a, Yikui Zhang
    Pages 141-146

  17. High-Throughput Binocular Pattern Electroretinograms in the Mouse

    • Tsung-Han Chou, Jonathon Toft-Nielsen, Vittorio Porciatti
    Pages 147-153

  18. Intravitreal Injection of AAV for the Transduction of Mouse Retinal Ganglion Cells

    • Bart Nieuwenhuis, Andrew Osborne
    Pages 155-174

  19. Methods to Identify Rat and Mouse Retinal Ganglion Cells in Retinal Flat-Mounts

    • Juan A. Miralles de Imperial-Ollero, Beatriz Vidal-Villegas, Alejandro Gallego-Ortega, Francisco M. Nadal-Nicolás, Manuel Salinas-Navarro, María Norte-Muñoz et al.
    Pages 175-194

  20. Back Matter

    Pages 195-196
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Editors and Affiliations

  • School of Optometry and Vision Sciences, Cardiff University, Cardiff, UK

    Ben Mead

Bibliographic Information

  • Book Title: Retinal Ganglion Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Ben Mead

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3409-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-3408-0Published: 10 August 2023

  • Softcover ISBN: 978-1-0716-3411-0Due: 10 September 2023

  • eBook ISBN: 978-1-0716-3409-7Published: 09 August 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 196

  • Number of Illustrations: 9 b/w illustrations, 49 illustrations in colour

  • Topics: Neurosciences, Anatomy

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