Skip to main content

7 Result(s)

within Yuanming Gai Remove this filter

and

  1. Article

    Open Access

    Protein engineering and iterative multimodule optimization for vitamin B6 production in Escherichia coli

    Vitamin B6 is an essential nutrient with extensive applications in the medicine, food, animal feed, and cosmetics industries. Pyridoxine (PN), the most common commercial form of vitamin B6, is currently chemicall...

    Linxia Liu, **long Li, Yuanming Gai, Zhizhong Tian, Yanyan Wang… in Nature Communications (2023)

    Download PDF (2025 KB) View Article

  2. No Access

    Article

    A multistrategy approach for improving the expression of E. coli phytase in Pichia pastoris

    Phytase is an additive in animal feed that degrades phytic acid in plant material, reducing feeding costs, and pollution from fecal phosphorus excretion. A multistrategy approach was adopted to improve the exp...

    Yuankun Helian, Yuanming Gai, Huan Fang… in Journal of Industrial Microbiology & Biote… (2020)

  3. No Access

    Article

    Metabolic engineering and optimization of the fermentation medium for vitamin B12 production in Escherichia coli

    Vitamin B12 is a crucial fine chemical that is widely used in the pharmaceutical, food and chemical industries, and its production solely dependents on microbial fermentation. We previously constructed an artific...

    Dong Li, Huan Fang, Yuanming Gai, **g Zhao… in Bioprocess and Biosystems Engineering (2020)

  4. Article

    Open Access

    A food-grade expression system for d-psicose 3-epimerase production in Bacillus subtilis using an alanine racemase-encoding selection marker

    Food-grade expression systems require that the resultant strains should only contain materials from food-safe microorganisms, and no antibiotic resistance marker can be utilized. To develop a food-grade expres...

    **gqi Chen, Zhaoxia **, Yuanming Gai, Jibin Sun… in Bioresources and Bioprocessing (2017)

    Download PDF (1527 KB) View Article

  5. Article

    Open Access

    Combinatorial Sec pathway analysis for improved heterologous protein secretion in Bacillus subtilis: identification of bottlenecks by systematic gene overexpression

    Secretory expression of valuable proteins by B. subtilis and its related species has attracted intensive work over the past three decades. Although very high yields can be achieved with homologous proteins, produ...

    **gqi Chen, Gang Fu, Yuanming Gai, ** Zheng, Dawei Zhang… in Microbial Cell Factories (2015)

    Download PDF (5725 KB) View Article

  6. No Access

    Article

    Enhanced extracellular production of α-amylase in Bacillus subtilis by optimization of regulatory elements and over-expression of PrsA lipoprotein

    α-Amylase was used as a heterologous model protein to investigate the effects of promoters, signal peptides and over-expression of an extra-cytoplasmic molecular chaperone, PrsA lipoprotein, on enhancing the s...

    **gqi Chen, Yuanming Gai, Gang Fu, Wenjuan Zhou, Dawei Zhang… in Biotechnology Letters (2015)

  7. Article

    Open Access

    Comparative functional analysis of Jembrana disease virus Tat protein on lentivirus long terminal repeat promoters: evidence for flexibility at its N-terminus

    Jembrana disease virus (JDV) encodes a potent regulatory protein Tat that strongly stimulates viral expression by transactivating the long terminal repeat (LTR) promoter. JDV Tat (jTat) promotes the transcript...

    Yang Su, Gang Deng, Yuanming Gai, Yue Li, Yang Gao, Jiansen Du… in Virology Journal (2009)

    Download PDF (2782 KB) View Article