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Open AccessThree-dimensional structured illumination microscopy with enhanced axial resolution
The axial resolution of three-dimensional structured illumination microscopy (3D SIM) is limited to ∼300 nm. Here we present two distinct, complementary methods to improve axial resolution in 3D SIM with minim...
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Article
Practical considerations for quantitative light sheet fluorescence microscopy
Fluorescence microscopy has evolved from a purely observational tool to a platform for quantitative, hypothesis-driven research. As such, the demand for faster and less phototoxic imaging modalities has spurre...
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Article
Open AccessIncorporating the image formation process into deep learning improves network performance
We present Richardson–Lucy network (RLN), a fast and lightweight deep learning method for three-dimensional fluorescence microscopy deconvolution. RLN combines the traditional Richardson–Lucy iteration with a ...
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Article
Three-dimensional residual channel attention networks denoise and sharpen fluorescence microscopy image volumes
We demonstrate residual channel attention networks (RCAN) for the restoration and enhancement of volumetric time-lapse (four-dimensional) fluorescence microscopy data. First we modify RCAN to handle image volu...
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Article
Rapid image deconvolution and multiview fusion for optical microscopy
The contrast and resolution of images obtained with optical microscopes can be improved by deconvolution and computational fusion of multiple views of the same sample, but these methods are computationally exp...
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Author Correction: Faster, sharper, and deeper: structured illumination microscopy for biological imaging
In the version of this Perspective originally published, Fig. 4g included an incorrect inset adapted from a different figure than the main image in the panel. This error has been corrected in the PDF and HTML ...
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Faster, sharper, and deeper: structured illumination microscopy for biological imaging
Structured illumination microscopy (SIM) allows rapid, super-resolution (SR) imaging in live specimens. We review recent technical advances in SR-SIM, with emphasis on imaging speed, resolution, and depth. Sin...
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Single-shot super-resolution total internal reflection fluorescence microscopy
We combined instant structured illumination microscopy (iSIM) with total internal reflection fluorescence microscopy (TIRFM) in an approach referred to as instant TIRF-SIM, thereby improving the lateral spatia...
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Adaptive optics improves multiphoton super-resolution imaging
Adaptive optics and two-photon instant structured illumination microscopy are combined to provide improved super-resolution imaging within optically aberrating biological samples.
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Open AccessWormGUIDES: an interactive single cell developmental atlas and tool for collaborative multidimensional data exploration
Imaging and image analysis advances are yielding increasingly complete and complicated records of cellular events in tissues and whole embryos. The ability to follow hundreds to thousands of cells at the indiv...
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Dual-view plane illumination microscopy for rapid and spatially isotropic imaging
Kumar et al. describe how to build a diSPIM from commercially available parts and use it to live-image cultured cells or worm embryogenesis. The inverted setup enables samples to be mounted directly on microscope...
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Spatially isotropic four-dimensional imaging with dual-view plane illumination microscopy
A dual-view light sheet microscope allows high-speed imaging with an isotropic spatial resolution