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Article
Open AccessA radiomics-boosted deep-learning for risk assessment of synchronous peritoneal metastasis in colorectal cancer
Synchronous colorectal cancer peritoneal metastasis (CRPM) has a poor prognosis. This study aimed to create a radiomics-boosted deep learning model by PET/CT image for risk assessment of synchronous CRPM.
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Open AccessThree-dimensional structured illumination microscopy with enhanced axial resolution
The axial resolution of three-dimensional structured illumination microscopy (3D SIM) is limited to ∼300 nm. Here we present two distinct, complementary methods to improve axial resolution in 3D SIM with minim...
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Article
Practical considerations for quantitative light sheet fluorescence microscopy
Fluorescence microscopy has evolved from a purely observational tool to a platform for quantitative, hypothesis-driven research. As such, the demand for faster and less phototoxic imaging modalities has spurre...
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Article
Open AccessIncorporating the image formation process into deep learning improves network performance
We present Richardson–Lucy network (RLN), a fast and lightweight deep learning method for three-dimensional fluorescence microscopy deconvolution. RLN combines the traditional Richardson–Lucy iteration with a ...
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Article
Open AccessMultiscale fluorescence imaging of living samples
Fluorescence microscopy is a highly effective tool for interrogating biological structure and function, particularly when imaging across multiple spatiotemporal scales. Here we survey recent innovations and ap...
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Article
Open AccessCytonemes coordinate asymmetric signaling and organization in the Drosophila muscle progenitor niche
Asymmetric signaling and organization in the stem-cell niche determine stem-cell fates. Here, we investigate the basis of asymmetric signaling and stem-cell organization using the Drosophila wing-disc that create...
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Multiview confocal super-resolution microscopy
Confocal microscopy1 remains a major workhorse in biomedical optical microscopy owing to its reliability and flexibility in imaging various samples, but suffers from substantial point spread function anisotropy, ...
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Article
Three-dimensional residual channel attention networks denoise and sharpen fluorescence microscopy image volumes
We demonstrate residual channel attention networks (RCAN) for the restoration and enhancement of volumetric time-lapse (four-dimensional) fluorescence microscopy data. First we modify RCAN to handle image volu...
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Article
Structural and developmental principles of neuropil assembly in C. elegans
Neuropil is a fundamental form of tissue organization within the brain1, in which densely packed neurons synaptically interconnect into precise circuit architecture2,3. However, the structural and developmental p...
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Article
Rapid image deconvolution and multiview fusion for optical microscopy
The contrast and resolution of images obtained with optical microscopes can be improved by deconvolution and computational fusion of multiple views of the same sample, but these methods are computationally exp...
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Author Correction: Faster, sharper, and deeper: structured illumination microscopy for biological imaging
In the version of this Perspective originally published, Fig. 4g included an incorrect inset adapted from a different figure than the main image in the panel. This error has been corrected in the PDF and HTML ...
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Faster, sharper, and deeper: structured illumination microscopy for biological imaging
Structured illumination microscopy (SIM) allows rapid, super-resolution (SR) imaging in live specimens. We review recent technical advances in SR-SIM, with emphasis on imaging speed, resolution, and depth. Sin...
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Single-shot super-resolution total internal reflection fluorescence microscopy
We combined instant structured illumination microscopy (iSIM) with total internal reflection fluorescence microscopy (TIRFM) in an approach referred to as instant TIRF-SIM, thereby improving the lateral spatia...
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Article
Open AccessReflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy
Light-sheet fluorescence microscopy (LSFM) enables high-speed, high-resolution, and gentle imaging of live specimens over extended periods. Here we describe a technique that improves the spatiotemporal resolut...
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Article
Adaptive optics improves multiphoton super-resolution imaging
Adaptive optics and two-photon instant structured illumination microscopy are combined to provide improved super-resolution imaging within optically aberrating biological samples.
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Open AccessWormGUIDES: an interactive single cell developmental atlas and tool for collaborative multidimensional data exploration
Imaging and image analysis advances are yielding increasingly complete and complicated records of cellular events in tissues and whole embryos. The ability to follow hundreds to thousands of cells at the indiv...
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Article
Dual-view plane illumination microscopy for rapid and spatially isotropic imaging
Kumar et al. describe how to build a diSPIM from commercially available parts and use it to live-image cultured cells or worm embryogenesis. The inverted setup enables samples to be mounted directly on microscope...
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Article
Spatially isotropic four-dimensional imaging with dual-view plane illumination microscopy
A dual-view light sheet microscope allows high-speed imaging with an isotropic spatial resolution
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Article
Cell-based biosensors: Towards the development of cellular monitoring
Cell-based biosensors (CBBs), a research hotspot of biosensors, which treat living cells as sensing elements, can detect the functional information of biologically active analytes. They characterize with high ...
