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Protocol
Detection of Protein Tyrosine Phosphatase Interacting Partners by Mass Spectrometry
Unraveling interacting partners of protein tyrosine (Tyr) phosphatases is considered a key aspect in resolving the regulation of signaling cascades either in a pathological or in developmental context. Mass sp...
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Article
Single-shot super-resolution total internal reflection fluorescence microscopy
We combined instant structured illumination microscopy (iSIM) with total internal reflection fluorescence microscopy (TIRFM) in an approach referred to as instant TIRF-SIM, thereby improving the lateral spatia...
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Article
Open AccessReflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy
Light-sheet fluorescence microscopy (LSFM) enables high-speed, high-resolution, and gentle imaging of live specimens over extended periods. Here we describe a technique that improves the spatiotemporal resolut...
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Article
Dual-view plane illumination microscopy for rapid and spatially isotropic imaging
Kumar et al. describe how to build a diSPIM from commercially available parts and use it to live-image cultured cells or worm embryogenesis. The inverted setup enables samples to be mounted directly on microscope...
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Article
Instant super-resolution imaging in live cells and embryos via analog image processing
An analog implementation of structured illumination using matched microlens and pinhole arrays allows up to 100-Hz 3D two-color imaging with 145-nm lateral and 350-nm axial resolution.
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Article
Compromise in mRNA processing machinery in senescent human fibroblasts: implications for a novel potential role of Phospho-ATR (ser428)
Ataxia-Telangiectasia and Rad3 related kinase (ATR) is a major gatekeeper of genomic stability and has been the subject of exhaustive study in the context of cell cycle progression and senescence as a DNA dama...
