14 Result(s)
within
Yevgeny Berdichevsky
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Article
Open AccessMicrodevice for directional axodendritic connectivity between micro 3D neuronal cultures
Neuronal cultures are widely used in neuroscience research. However, the randomness of circuits in conventional cultures prevents accurate in vitro modeling of cortical development and of the pathogenesis of n...
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Article
Open AccessNeuron and astrocyte aggregation and sorting in three-dimensional neuronal constructs
Aggregation and self-sorting of cells in three dimensional cultures have been described for non-neuronal cells. Despite increased interest in engineered neural tissues for treating brain injury or for modeling...
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Article
Open AccessDeubiquitylating enzymes in neuronal health and disease
Ubiquitylation and deubiquitylation play a pivotal role in protein homeostasis (proteostasis). Proteostasis shapes the proteome landscape in the human brain and its impairment is linked to neurodevelopmental a...
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Protocol
Protocol for Rodent Organotypic Hippocampal Slice Culture Model for Ex Vivo Monitoring of Epileptogenesis
Brain injury is one of the common causes of acquired epilepsy. Current treatments include anti-epileptic drugs that are anticonvulsant. However, around 30% of patients become resistant. Better understanding of...
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Article
Open AccessNeuroprotective levels of IGF-1 exacerbate epileptogenesis after brain injury
Exogenous Insulin-Like Growth Factor-1 (IGF-1) is neuroprotective in animal models of brain injury, and has been considered as a potential therapeutic. Akt-mTOR and MAPK are downstream targets of IGF-1 signali...
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Article
Perfused drop microfluidic device for brain slice culture-based drug discovery
Living slices of brain tissue are widely used to model brain processes in vitro. In addition to basic neurophysiology studies, brain slices are also extensively used for pharmacology, toxicology, and drug discove...
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Article
Multi-electrode array capable of supporting precisely patterned hippocampal neuronal networks
Accurate positioning of primary mouse hippocampal neurons on electrodes enables the recording from and stimulation of specified individual neurons on a multi-electrode array (MEA). In this work, positive diele...
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Article
Spike bursts increase amyloid-β 40/42 ratio by inducing a presenilin-1 conformational change
Relative amounts of amyloid-β peptides of different length are altered in the brains of Alzheimer's disease patients. Here the authors show that neuronal patterns differentially regulate the production of amyl...
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Article
Nanoporous Gold: A Biomaterial for Microfabricated Drug-Delivery Platforms
Nanoporous gold (np-Au) is a promising nanostructured material with many desirable properties, including large surface area-to-volume ratio, corrosion resistance, high conductivity, and well-studied thiol-base...
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Article
Polymer Microvalve Based on Anisotropic Expansion of Polypyrrole
An actuator fabricated from the conductive polymer polypyrrole using microfabrication techniques is presented. This actuator utilizes the anisotropic volume change, which occurs under application of an electri...
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Article
Open AccessPropofol induces MAPK/ERK cascade dependant expression of cFos and Egr-1 in rat hippocampal slices
Propofol is a commonly used intravenous anesthetic agent, which produce rapid induction of and recovery from general anesthesia. Numerous clinical studies reported that propofol can potentially cause amnesia a...
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Protocol
Cell-Free Assays
The superoxide (O2 •−)-generating enzyme complex of phagocytes, known as the NADPH oxidase, can be assayed in a number of in vitro cell-free (or broken cell) systems. These consist of a mixture of...
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Article
Fabrication and Evaluation of Conducting Polymer Nanowire Heterostructures
Conducting polymer nanostructures such as nanofibers and nanotubes have potential uses in a variety of applications including electronic and photonic devices and sensors. Conducting polymers have also been use...
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Protocol
Large Scale Production of Recombinant Antibodies by Utilizing Cellulose-Binding Domains
Many recombinant proteins, particularly proteins with diagnostic and therapeutic potential such as antibodies, lymphokines, receptors, enzymes, and enzyme-inhibitors, are being produced from transformed host c...
