13 Result(s)
within
Xun **a
Physics
Biochemistry, general
Spectroscopy/Spectrometry
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Article
Distinct stereospecific effect of chiral tether between a tag and protein on the rigidity of paramagnetic tag
Flexibility between the paramagnetic tag and its protein conjugates is a common yet unresolved issue in the applications of paramagnetic NMR spectroscopy in biological systems. The flexibility greatly attenuat...
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Article
Stable and rigid DTPA-like paramagnetic tags suitable for in vitro and in situ protein NMR analysis
Organic synthesis of a ligand with high binding affinities for paramagnetic lanthanide ions is an effective way of generating paramagnetic effects on proteins. These paramagnetic effects manifested in high-res...
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Article
Site-specific tagging proteins with a rigid, small and stable transition metal chelator, 8-hydroxyquinoline, for paramagnetic NMR analysis
Design of a paramagnetic metal binding motif in a protein is a valuable way for understanding the function, dynamics and interactions of a protein by paramagnetic NMR spectroscopy. Several strategies have been...
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Article
Selective 15N-labeling of the side-chain amide groups of asparagine and glutamine for applications in paramagnetic NMR spectroscopy
The side-chain amide groups of asparagine and glutamine play important roles in stabilizing the structural fold of proteins, participating in hydrogen-bonding networks and protein interactions. Selective 15N-labe...
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Article
Erratum to: Suppression of isotope scrambling in cell-free protein synthesis by broadband inhibition of PLP enymes for selective 15N-labelling and production of perdeuterated proteins in H2O
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Article
Engineering [Ln(DPA)3]3− binding sites in proteins: a widely applicable method for tagging proteins with lanthanide ions
Paramagnetic relaxation enhancements from unpaired electrons observed in nuclear magnetic resonance (NMR) spectra present powerful long-range distance restraints. The most frequently used paramagnetic tags, ho...
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Article
Erratum to: Paramagnetic labelling of proteins and oligonucleotides for NMR
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Article
Suppression of isotope scrambling in cell-free protein synthesis by broadband inhibition of PLP enymes for selective 15N-labelling and production of perdeuterated proteins in H2O
Selectively isotope labelled protein samples can be prepared in vivo or in vitro from selectively labelled amino acids but, in many cases, metabolic conversions between different amino acids result in isotope ...
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Article
Tunable paramagnetic relaxation enhancements by [Gd(DPA)3]3− for protein structure analysis
Paramagnetic relaxation enhancements (PRE) present a powerful source of structural information in nuclear magnetic resonance (NMR) studies of proteins and protein–ligand complexes. In contrast to conventional ...
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Article
Paramagnetic labelling of proteins and oligonucleotides for NMR
Paramagnetic effects offer a rich source of long-range structural restraints. Here we review current methods for site-specific tagging of proteins and oligonucleotides with paramagnetic molecules. The paramagn...
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Article
Numbat: an interactive software tool for fitting Δχ-tensors to molecular coordinates using pseudocontact shifts
Pseudocontact shift (PCS) effects induced by a paramagnetic lanthanide bound to a protein have become increasingly popular in NMR spectroscopy as they yield a complementary set of orientational and long-range ...
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Article
Measurement of dissociation constants of high-molecular weight protein–protein complexes by transferred 15N-relaxation
The use of 15N-relaxation data for determination of the dissociation constant of a protein–protein complex is proposed for the situation where a 15N-labeled protein is bound to an unlabeled protein of high molecu...
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Article
Amino-acid Type Identification in 15N-HSQC Spectra by Combinatorial Selective 15N-labelling
The efficiency of cell-free protein synthesis combined with combinatorial selective 15N-labelling provides a method for the rapid assignment of 15N-HSQC cross-peaks to the 19 different non-proline amino-acid type...
