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  1. No Access

    Protocol

    Preparation of Multiple Site-Specific Mutant Proteins for NMR Studies by PCR-Directed Cell-Free Protein Synthesis

    Cell-free protein synthesis (CFPS) offers a fast and inexpensive approach to selectively label proteins with isotopes that can then be detected by nuclear magnetic resonance (NMR) spectroscopy directly in the ...

    Kiyoshi Ozawa, Ruhu Qi in Cell-Free Protein Synthesis (2014)

  2. Article

    Erratum to: Suppression of isotope scrambling in cell-free protein synthesis by broadband inhibition of PLP enymes for selective 15N-labelling and production of perdeuterated proteins in H2O

    Xun-Cheng Su, Choy-Theng Loh, Ruhu Qi, Gottfried Otting in Journal of Biomolecular NMR (2011)

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  3. No Access

    Article

    Suppression of isotope scrambling in cell-free protein synthesis by broadband inhibition of PLP enymes for selective 15N-labelling and production of perdeuterated proteins in H2O

    Selectively isotope labelled protein samples can be prepared in vivo or in vitro from selectively labelled amino acids but, in many cases, metabolic conversions between different amino acids result in isotope ...

    Xun-Cheng Su, Choy-Theng Loh, Ruhu Qi, Gottfried Otting in Journal of Biomolecular NMR (2011)