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  1. Article

    Open Access

    Activation of L-lactate oxidase by the formation of enzyme assemblies through liquid–liquid phase separation

    The assembly state of enzymes is gaining interest as a mechanism for regulating the function of enzymes in living cells. One of the current topics in enzymology is the relationship between enzyme activity and ...

    Tomoto Ura, Ako Kagawa, Nanako Sakakibara, Hiromasa Yagi… in Scientific Reports (2023)

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    Article

    A systematic study of labelling an α-helix in a protein with a lanthanide using IDA-SH or NTA-SH tags

    The previously published IDA-SH and NTA-SH tags are small synthetic lanthanide-binding tags derived from cysteine, which afford site-specific lanthanide labelling by disulfide-bond formation with a cysteine re...

    Hiromasa Yagi, Ansis Maleckis, Gottfried Otting in Journal of Biomolecular NMR (2013)

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    Article

    Engineering [Ln(DPA)3]3− binding sites in proteins: a widely applicable method for tagging proteins with lanthanide ions

    Paramagnetic relaxation enhancements from unpaired electrons observed in nuclear magnetic resonance (NMR) spectra present powerful long-range distance restraints. The most frequently used paramagnetic tags, ho...

    **nying Jia, Hiromasa Yagi, Xun-Cheng Su… in Journal of Biomolecular NMR (2011)

  4. No Access

    Article

    Tunable paramagnetic relaxation enhancements by [Gd(DPA)3]3− for protein structure analysis

    Paramagnetic relaxation enhancements (PRE) present a powerful source of structural information in nuclear magnetic resonance (NMR) studies of proteins and protein–ligand complexes. In contrast to conventional ...

    Hiromasa Yagi, Karin V. Loscha, Xun-Cheng Su… in Journal of Biomolecular NMR (2010)

  5. No Access

    Article

    Dynamic inter-subunit interactions in thermophilic F1-ATPase subcomplexes studied by cross-correlated relaxation-enhanced polarization transfer NMR

    F1-ATPase is a unique enzyme in terms of its rotational catalytic activity. The smallest unit showing this property is the α3β3γ complex (351 kDa). For investigation of such a huge system by means of solution NMR...

    Masumi Kobayashi, Hiromasa Yagi, Toshio Yamazaki… in Journal of Biomolecular NMR (2008)

  6. No Access

    Article

    Contributory presentations/posters

    N. Manoj, V. R. Srinivas, A. Surolia, M. Vijayan, K. Suguna… in Journal of Biosciences (1999)