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Protocol
Disaggregation and Reaggregation of Zebrafish Retinal Cells for the Analysis of Neuronal Layering
The reaggregation of dissociated cells to form organotypic structures provides an in vitro system for the analysis of the cellular interactions and molecular mechanisms involved in the formation of tissue arch...
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Article
Open AccessErratum to: Differential requirement of F-actin and microtubule cytoskeleton in cue-induced local protein synthesis in axonal growth cones
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Article
Open AccessDorsoventral patterning of the Xenopus eye involves differential temporal changes in the response of optic stalk and retinal progenitors to Hh signalling
Hedgehog (Hh) signals are instrumental to the dorsoventral patterning of the vertebrate eye, promoting optic stalk and ventral retinal fates and repressing dorsal retinal identity. There has been limited analy...
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Open AccessDifferential requirement of F-actin and microtubule cytoskeleton in cue-induced local protein synthesis in axonal growth cones
Local protein synthesis (LPS) via receptor-mediated signaling plays a role in the directional responses of axons to extrinsic cues. An intact cytoskeleton is critical to enact these responses, but it is not kn...
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Article
Coupling of NF-protocadherin signaling to axon guidance by cue-induced translation
This study shows that a non-clustered protocadherin, NFPC, is locally translated in retinal axons in response to Sema3A and demonstrates that NFPC-mediated homophilic adhesion helps guide retinal axons in the ...
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Article
Metabolic differentiation in the embryonic retina
It is unclear whether proliferating and differentiating cells produce energy through different metabolic pathways. Harris and colleagues show, in the embryonic Xenopus retina, that dividing progenitors use glycog...
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Article
Open AccessPtf1a is expressed transiently in all types of amacrine cells in the embryonic zebrafish retina
The vertebrate retina is composed of five major types of neurons: three excitatory (photoreceptors, bipolar cells and ganglion cells) and two inhibitory (horizontal and amacrine cells). The transcription facto...
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Article
Open AccessNon-SMC condensin I complex proteins control chromosome segregation and survival of proliferating cells in the zebrafish neural retina
The condensation of chromosomes and correct sister chromatid segregation during cell division is an essential feature of all proliferative cells. Structural maintenance of chromosomes (SMC) and non-SMC proteins f...
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Open AccessVsx2 in the zebrafish retina: restricted lineages through derepression
The neurons in the vertebrate retina arise from multipotent retinal progenitor cells (RPCs). It is not clear, however, which progenitors are multipotent or why they are multipotent.
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Article
Open AccessCep70 and Cep131 contribute to ciliogenesis in zebrafish embryos
The centrosome is the cell's microtubule organising centre, an organelle with important roles in cell division, migration and polarity. However, cells can divide and flies can, for a large part of development,...
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Open AccessPolarization and orientation of retinal ganglion cells in vivo
In the absence of external cues, neurons in vitro polarize by using intrinsic mechanisms. For example, cultured hippocampal neurons extend arbitrarily oriented neurites and then one of these, usually the one near...
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Mechanisms of ventral patterning in the vertebrate nervous system
Lupo and colleagues review the crucial molecular pathways that regulate ventral patterning of the telencephalon, eye and spinal cord, and show that ventral patterning can be explained by a few basic molecular ...
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Endocytosis-dependent desensitization and protein synthesis–dependent resensitization in retinal growth cone adaptation
It has been proposed that growth cones navigating through gradients adapt to baseline concentrations of guidance cues. This adaptation process is poorly understood. Using the collapse assay, we show that adapt...
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Specifying motor neurons: up and down and back to front
A recent study in Nature shows that the columnar fate of motor neurons in the embryonic spinal cord is imposed by cross-repressive patterns of Hox-c expression. This Hox expression is in turn controlled by graded...
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Article
Clocking the birth of neurons
Different types of neurons are born in a conserved, sequential order during development. The molecular cogs in the clock-like mechanism driving this process are now being revealed.
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Article
Seminal plasma trace metal levels in industrial workers
This study compares the seminal plasma trace metal levels of hospital workers with groups of industrial workers in a petroleum refinery, smelter, and chemical plant. The metals measured were the essential meta...
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Connecting the Eye with the Brain: The Formation of the Retinotectal Pathway
The retino-tectal system has served as a model for the growth and navigation of axons in the develo** brain for over 50 years, when the first anatomical studies of embryos revealed details of the ontogeny of...
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Article
A critical window for cooperation and competition among develo** retinotectal synapses
In the develo** frog visual system, topographic refinement of the retinotectal projection depends on electrical activity. In vivo whole-cell recording from develo** Xenopus tectal neurons shows that convergen...
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Comparison of sperm viability with seminal plasma metal levels
This study compares the semen levels of lead (Pb), cadmium (Cd), and aluminum (Al) in relation to live sperm in semen samples from 64 apparently healthy men. The measured levels were separated into live sperm ...
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Regulation of neuronal diversity in the Xenopus retina by Delta signalling
To generate the variety of mature neurons and glia found in the develo** retina, the competence of pluripotent progenitor cells to respond to extracellular signals must be controlled. Delta, a ligand of the...
