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Article
Heritable DNA-free genome editing of canola (Brassica napus L.) using PEG-mediated transfection of isolated protoplasts
Application of new genome editing technologies for plant improvement depends on efficient delivery of editing components into plant cells. For editing of canola, which is an important oil crop, CRISPR/Cas9 and Ag...
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Article
“Cell grafting”: a new approach for transferring cytoplasmic or nuclear genome between plants
A new method based on mixing and wounding of callus tissue was used to transfer plastid or nuclear DNA between cells.
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Protocol
Agrobacterium-Mediated Maize Transformation: Immature Embryos Versus Callus
Transformation with Agrobacterium tumefaciens is the preferred method for delivery of transgenes into a wide range of plant species including maize. Optimized protocols for the Agrobacterium-mediated transformati...
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Article
Agrobacterium-mediated transformation of seedling-derived maize callus
Efficient production of seedling-derived Type I callus was demonstrated for several corn genotypes including commercial inbred lines. Seeds were germinated on MS-based medium containing 10 mg l−1 picloram and 3 m...