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Article
Synthetic p72 Peptide-Based Indirect- Enzyme-Linked Immunosorbent Assay for Diagnosis of African Swine Fever Virus Infection
In the present study, we explored the potential diagnostic application of B cell linear peptides derived from the p72 protein of African Swine Fever Virus (ASFV) using a two-step bioinformatics approach for de...
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Article
Apoptin NLS2 homodimerization strategy for improved antibacterial activity and bio-stability
The emergence of antibiotic resistance prompts exploration of viable antimicrobial peptides (AMPs) designs. The present study explores the antimicrobial prospects of Apoptin nuclear localization sequence (NLS2...
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Article
Development of B cell epitopes-based enzyme linked immune sorbent assay for detection of bovine anti-Mullerian hormone
The present study aimed to generate antibodies against predicted B cell epitopic peptides encoding bAMH for develo** different ELISA models. Sandwich ELISA was determined to be an excellent technique for ass...
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Chapter
Point-of-Care Diagnostics Using Molecular Approaches
Molecular point care diagnostics aimed to provide simplified diagnostic platforms which are rapid and designed in miniaturized forms for on-field disease diagnosis. There are various approaches to develop mole...
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Protocol
Antibody-Based Sensors for Pathogen Detection
Antibodies are soluble biomolecules of the Immunoglobulin family found in serum, which can specifically bind to and neutralize diverse antigens. Since their discovery, antibodies have been utilized for diagnos...
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Protocol
Peptide Nucleic Acid (PNA): A Diagnostic Molecule for Infectious Diseases
Disease diagnosis performs an important role in the epidemiology of the disease. Genetic material identification is the definite way of identification of the pathogen. Available techniques involved in genetic ...
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Article
Novel peptide (RATH) mediated delivery of peptide nucleic acids for antiviral interventions
The peptide nucleic acid (PNA) is a chimeric molecule with the nucleobases connected by peptide bonds. This chimeric nature gives the PNA certain therapeutic advantages over natural antisense nucleic acid mole...
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Article
A bio-safe multiple antigenic peptide (MAP) enzyme-linked immunoassay for the detection of antibodies to infectious bronchitis virus in chickens
The objective of the study was to develop a bio-safe synthetic peptide ELISA for the detection of antibodies against the infectious bronchitis virus (IBV) using a novel multiple antigenic peptide approach (MAP...