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Article
Acaricide resistance status of deltamethrin and coumaphos in Hyalomma anatolicum ticks collected from different districts of Haryana
To study the acaricide resistance status and possible mechanisms of action in conferring resistance to commonly used acaricides (deltamethrin and coumaphos), Hyalomma anatolicum ticks were collected from 6 dairy ...
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Article
Novel Isothermal Reverse Transcription Polymerase Spiral Reaction (RT-PSR) Assay for the Detection of Newcastle Disease Virus in Avian Species
Newcastle disease in avian species is caused by Newcastle disease virus (NDV). It is responsible for large economic losses to the poultry industry. The early diagnosis of NDV infection is important for epidemi...
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Article
Rapid detection of porcine sapelovirus by reverse transcription recombinase polymerase amplification assay
Porcine Sapelovirus (PSV) infection has been confirmed in pigs worldwide, mostly asymptomatic, but in some cases, it can lead to significant issues in the gastrointestinal, respiratory, neurological, or reprod...
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Article
Apoptin NLS2 homodimerization strategy for improved antibacterial activity and bio-stability
The emergence of antibiotic resistance prompts exploration of viable antimicrobial peptides (AMPs) designs. The present study explores the antimicrobial prospects of Apoptin nuclear localization sequence (NLS2...
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Article
Temporal analysis of circulating nucleic acid in early days of pregnancy in buffalo
Improving reproductive efficiency in livestock relies mainly on the ability to detect pregnancy quickly and accurately. Recently, circulating nucleic acids (CNAs) have been exploited for prenatal diagnosis in ...
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Chapter
Point-of-Care Diagnostics Using Molecular Approaches
Molecular point care diagnostics aimed to provide simplified diagnostic platforms which are rapid and designed in miniaturized forms for on-field disease diagnosis. There are various approaches to develop mole...
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Article
Isolation and Molecular Characterization of MDV-1 from Vaccinated Poultry Birds in Haryana State of India
Marek’s disease (MD) is a lymphoproliferative and neuropathic disease of domestic fowl caused by an oncogenic Gallid Herpesvirus-2 (GaHV-2), also known as Marek’s Disease Virus serotype 1 (MDV-1). A total of f...
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Protocol
Antibody-Based Sensors for Pathogen Detection
Antibodies are soluble biomolecules of the Immunoglobulin family found in serum, which can specifically bind to and neutralize diverse antigens. Since their discovery, antibodies have been utilized for diagnos...
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Protocol
Peptide Nucleic Acid (PNA): A Diagnostic Molecule for Infectious Diseases
Disease diagnosis performs an important role in the epidemiology of the disease. Genetic material identification is the definite way of identification of the pathogen. Available techniques involved in genetic ...
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Article
End Point Multiplex PCR for Diagnosis of Haemoprotozoan Diseases in Cattle
Theileriosis, trypanosomosis and babesiosis are the three major haemoprotozoan diseases causing huge economic losses worldwide. Difficulty in diagnosis of these diseases lies with the detection of carrier stat...
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Article
Open AccessVirological, immunological and pathological findings of transplacentally transmitted bluetongue virus serotype 1 in IFNAR1-blocked mice during early and mid gestation
Transplacental transmission (TPT) of wild-type Indian BTV-1 had never been experimentally proved. This study was first time investigated TPT of Indian BTV-1 (isolated from aborted and stillborn goat fetal sple...
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Chapter
Orbiviruses
The family Reoviridae contains 15 genera with the genus Orbivirus being the largest of the currently classified ones. It contains Bluetongue virus (the Orbivirus type species), 27 virus species with bluetongue vi...
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Article
Molecular Cloning and Characterization of Mx2 for Early Pregnancy Diagnosis in Bubalus bubalis
India is the largest milk producing country in the world. This major achievement is only possible due to the presence of one of the species Bubalus bubalis (Buffalo). Early pregnancy diagnosis in buffalo is one o...
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Article
Comparative study on hemato-biochemical alterations and selected acute phase protein response in native sheep experimentally infected with bluetongue virus serotypes 10 and 24
Bluetongue (BT) is an infectious, non-contagious, insect-transmitted viral disease of sheep, other domestic, and wild ruminants caused by bluetongue virus (BTV), a prototype species of the genus Orbivirus of the ...
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Article
Quantitative RT-PCR assays for identification and ty** of the Equine encephalosis virus
Equine encephalosis (EE) is an acute, arthropod-borne, noncontagious, febrile disease of equids. The clinical signs of EE are similar to milder forms of African horse sickness (AHS) and the two diseases can be...
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Chapter
Novel Molecular Diagnostics and Therapeutic Tools for Livestock Diseases
Recent novelties in diverse diagnostics and therapeutic tools in animal health sector have paved a brighter and clearer way ahead. These are proved to be better in detection, management, control and eradicatio...
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Chapter
Bluetongue: Aetiology, Epidemiology, Pathogenesis, Diagnosis and Control
Bluetongue (BT) is an emerging and re-emerging vector-borne viral disease of domestic and wild ruminants, caused by viruses classified within the species bluetongue virus (BTV), genus Orbivirus, family Reoviridae
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Article
Isolation and molecular characterization of contagious pustular dermatitis virus from Rajasthan, India
This article describes the isolation and identification of contagious pustular dermatitis virus/orf virus (ORFV) from an outbreak of contagious pustular dermatitis (orf) in flocks of goats, in the north wester...
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Article
Detection and molecular characterization of Newcastle disease virus in peafowl (Pavo cristatus) in Haryana State, India
Present study was undertaken to investigate the cause of deaths of peafowls in Haryana State. In total, 145 birds were sick and 28 birds were reported dead during July to September 2012. Some of the sick birds...
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Chapter
Whole Genome Sequencing Strategies and Development of Orbivirus Sequence Database: Implications for Novel dsRNA Virus Detection
The genus Orbivirus is the largest of the genera within the family Reoviridae, containing 22 recognised virus species as well as 15 unclassified ‘orbiviruses’, which could potentially represent further new specie...
