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Article
Comparative assessment of two in-house-built isothermal assays for visual detection of African swine fever virus
Owing to the lack of effective vaccines, current control measures and eradication strategies for the African swine fever virus (ASFV) rely on early detection and stringent stam**-out procedures. In the prese...
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Article
Characterization of an African swine fever virus outbreak in India and comparative analysis of immune genes in infected and surviving crossbreed vs. indigenous Doom pigs
Since 2020, African swine fever (ASF) has affected all pig breeds in Northeast India except Doom pigs, a unique indigenous breed from Assam and the closest relatives of Indian wild pigs. ASF outbreaks result i...
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Article
Natural co-infection of pigs with African swine fever virus and porcine reproductive and respiratory syndrome virus in India
Porcine reproductive and respiratory syndrome (PRRS) and African swine fever (ASF) are economically important diseases of pigs throughout the world. During an outbreak, all age groups of animals except piglets...
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Article
Transcriptome signatures of host tissue infected with African swine fever virus reveal differential expression of associated oncogenes
African swine fever (ASF) has emerged as a threat to swine production worldwide. Evasion of host immunity by ASF virus (ASFV) is well understood. However, the role of ASFV in triggering oncogenesis is still un...
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Article
Feasibility for Bioethanol Production from Poultry Excreta
The current study aimed to produce small-scale bioethanol from poultry excreta using traditional anerobic fermentation process. The predicted yield of the bioethanol was 1.47–2.16 gm/kg of commercial layer pou...
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Article
Development of triplex assay for simultaneous detection of Escherichia coli, methicillin resistant and sensitive Staphylococcus aureus in raw pork samples of retail markets
Escherichia coli and Staphylococcus aureus are the most important food borne pathogen transmitting from animal meat and meat products. Therefore, it is vital to design an accurate and specific diagnostic tool for...
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Chapter
Host-Directed Antiviral Therapy
Most of the antiviral drugs developed so far have been specifically designed to target particular pathogens and their unique viral pathways. However, due to their pathogen-specific nature, these drugs may not ...
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Article
Isolation and Characterization of Extended-Spectrum β-Lactamase Producing Escherichia coli from Pig Farms and Slaughterhouse
Extended-spectrum β-lactamase (ESBL) producing Escherichia coli represents a formidable challenge in the field of microbiology and public health due to its resistance to commonly used antibiotics. These strains p...
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Article
Development of B cell epitopes-based enzyme linked immune sorbent assay for detection of bovine anti-Mullerian hormone
The present study aimed to generate antibodies against predicted B cell epitopic peptides encoding bAMH for develo** different ELISA models. Sandwich ELISA was determined to be an excellent technique for ass...
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Article
Development of an on-site lateral flow immune assay based on mango leaf derived colloidal silver nanoparticles for rapid detection of Staphylococcus aureus in milk
In order to ensure food safety, screening food samples for the presence of pathogens has been categorised as a legal testing item throughout the globe. One of the most prevalent zoonotic bacteria transmitted t...
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Article
Profiling of key heat shock proteins and their relationship with male sexual behavior and seminal characteristics in Kankrej (Bos indicus) breeding bulls during different seasons
The goal of this study is to use indirect ELISA to determine the concentration of major heat shock proteins (Hsps) in Kankrej (Bos indicus) breeding bulls and their relationship with certain male phenotypic trait...
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Article
Development of CD163 receptor-based enzyme-linked immunosorbent assay for diagnosis of porcine reproductive and respiratory syndrome virus
Porcine reproductive and respiratory syndrome (PRRS) is an important economical disease in the global swine industry. The accurate detection of the PRRS virus (PRRSV) antigen is essential for the disease contr...
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Protocol
Lateral Flow Assay for Diagnosis of Pig Viral Diseases
Several infectious pig viral diseases of economic importance such as foot and mouth disease, Hog cholera, and African swine fever virus (ASFV) pose a serious threat to the swine industry worldwide. Control of ...
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Protocol
Production of Virus-like Particles Using the Baculovirus Expression System and Their Application in Vaccines and Viral Disease Diagnosis
Over many years, the baculovirus expression vector system (BEVS) has grown in popularity as a platform for expressing recombinant proteins. In addition to high levels of expression, the recombinant protein is ...
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Protocol
Multiplex PCR for Diagnosis of Porcine Diseases
Molecular diagnostics have revolutionized the efficiency of diagnosis of infectious diseases. An accurate diagnosis of a disease would prevent further infestation of infections in the healthy population. Altho...
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Protocol
Recombinant Antigen-Based Diagnostic Assays of Pig Viral Diseases
Recombinant DNA technology or genetic engineering is one of the most explored technologies in the current era as it is one of the convincing technologies for the detection of both antigen and antibody. Recombi...
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Article
Comparative miRNA signatures among Sahiwal and Frieswal cattle breeds during summer stress
MicroRNAs (miRNAs) are known to take part in different biological mechanisms, including biotic as well as abiotic cellular stresses. The present investigation was aimed to identify comparative expression profi...
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Article
Characterization of a putative ribosome binding site at the 5′ untranslated region of bovine heat shock protein 90
Untranslated regions (UTRs) of the transcripts play significant roles in translation regulation and continue to raise many intriguing questions in our understanding of cellular stress physiology. Internal ribo...
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Article
Identification of differentially expressed microRNAs in Sahiwal (Bos indicus) breed of cattle during thermal stress
microRNAs (miRNAs) are a class of small non-coding RNAs that play key roles in post transcriptional gene regulation that influence various fundamental cellular processes, including the cellular responses durin...
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Article
Open AccessT-ARMS PCR genoty** of SNP rs445709131 using thermostable strand displacement polymerase
In a recent publication, we reported the successful use of tetra primer-amplification refractory mutation system based polymerase chain reaction (T-ARMS-PCR) for genoty** of rs445709131-SNP responsible for t...
