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Protocol
Detection of Human Papillomaviruses by Polymerase Chain Reaction and In Situ Hybridization
The clinical utility of human papillomavirus (HPV) testing continues to be the focus of much debate. The clear epidemiologic link of HPV infection with the development of cervical intraepithelial neoplasia and...
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Article
Open AccessAnalysis of 16S rRNA gene sequences and circulating cell-free DNA from plasma of chronic fatigue syndrome and non-fatigued subjects
The association of an infectious agent with chronic fatigue syndrome (CFS) has been difficult and is further complicated by the lack of a known lesion or diseased tissue. Cell-free plasma DNA could serve as a ...
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Article
Open AccessBioelectronic DNA detection of human papillomaviruses using eSensor™: a model system for detection of multiple pathogens
We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor™) in identifying multiple pathogens.
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Article
Open AccessIntegration of gene expression, clinical, and epidemiologic data to characterize Chronic Fatigue Syndrome
Chronic fatigue syndrome (CFS) has no diagnostic clinical signs or diagnostic laboratory abnormalities and it is unclear if it represents a single illness. The CFS research case definition recommends stratifyi...
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Article
Open AccessIdentification of ambiguities in the 1994 chronic fatigue syndrome research case definition and recommendations for resolution
Chronic fatigue syndrome (CFS) is defined by symptoms and disability, has no confirmatory physical signs or characteristic laboratory abnormalities, and the etiology and pathophysiology remain unknown. Difficu...
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Protocol
Molecular Characterization of Human Papillomaviruses by PCR and In Situ Hybridization
The goal of early detection and screening is the diagnosis and treatment of cancer before it spreads beyond the organ of origin, perhaps even in its preinvasive state. Unfortunately, available early detection ...
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Article
Open AccessThe economic impact of chronic fatigue syndrome
Chronic fatigue syndrome (CFS) is a chronic incapacitating illness that affects between 400,000 and 800,000 Americans. Despite the disabling nature of this illness, scant research has addressed the economic im...
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Article
Differential-display PCR of peripheral blood for biomarker discovery in chronic fatigue syndrome
We used differential-display PCR of peripheral blood mononuclear cells (PBMCs) to search for candidate biomarkers for chronic fatigue syndrome (CFS). PBMCs were collected from a subject with CFS and an age- an...
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Article
Open AccessExercise responsive genes measured in peripheral blood of women with Chronic Fatigue Syndrome and matched control subjects
Chronic fatigue syndrome (CFS) is defined by debilitating fatigue that is exacerbated by physical or mental exertion. To search for markers of CFS-associated post-exertional fatigue, we measured peripheral blo...
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Article
Open AccessGene expression profile of cervical tissue compared to exfoliated cells: Impact on biomarker discovery
Exfoliated cervical cells are used in cytology-based cancer screening and may also be a source for molecular biomarkers indicative of neoplastic changes in the underlying tissue. However, because of keratiniza...
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Article
Open AccessEvaluation of autoantibodies to common and neuronal cell antigens in Chronic Fatigue Syndrome
People with chronic fatigue syndrome (CFS) suffer from multiple symptoms including fatigue, impaired memory and concentration, unrefreshing sleep and musculoskeletal pain. The exact causes of CFS are not known...
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Article
Open AccessChronic Fatigue Syndrome – A clinically empirical approach to its definition and study
The lack of standardized criteria for defining chronic fatigue syndrome (CFS) has constrained research. The objective of this study was to apply the 1994 CFS criteria by standardized reproducible criteria.
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Article
Open AccessPreliminary evidence of mitochondrial dysfunction associated with post-infective fatigue after acute infection with Epstein Barr Virus
Acute infectious diseases are typically accompanied by non-specific symptoms including fever, malaise, irritability and somnolence that usually resolve on recovery. However, in some individuals these symptoms ...
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Protocol
Microarray Technology for Use in Molecular Epidemiology
Microarrays are a powerful laboratory tool for the simultaneous assessment of the activity of thousands genes. Remarkable advances in biological sample collection, preparation and automation of hybridization h...
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Article
Open AccessInclusion of the glucocorticoid receptor in a hypothalamic pituitary adrenal axis model reveals bistability
The body's primary stress management system is the hypothalamic pituitary adrenal (HPA) axis. The HPA axis responds to physical and mental challenge to maintain homeostasis in part by controlling the body's co...
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Article
Open AccessComparison of target labeling methods for use with Affymetrix GeneChips
Several different commercial one-cycle labeling kits are available for preparation of the target for use with the Affymetrix GeneChip platform. However, there have been no evaluations of these different kits t...
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Article
Open AccessLaboratory methods to improve SELDI peak detection and quantitation
Protein profiling with surface-enhanced laser desorption-ionisation time-of-flight mass spectrometry (SELDI-TOF MS) is a promising approach for biomarker discovery. Some candidate biomarkers have been identifi...
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Article
Open AccessA method for improving SELDI-TOF mass spectrometry data quality
Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) is a powerful tool for rapidly generating high-throughput protein profiles from a large number of samples. However, ...
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Article
Open AccessEvidence of inflammatory immune signaling in chronic fatigue syndrome: A pilot study of gene expression in peripheral blood
Genomic profiling of peripheral blood reveals altered immunity in chronic fatigue syndrome (CFS) however interpretation remains challenging without immune demographic context. The object of this work is to ide...
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Article
Open AccessIntegrated Weighted Gene Co-expression Network Analysis with an Application to Chronic Fatigue Syndrome
Systems biologic approaches such as Weighted Gene Co-expression Network Analysis (WGCNA) can effectively integrate gene expression and trait data to identify pathways and candidate biomarkers. Here we show tha...
