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  1. Article

    Open Access

    Emerging microalgal feed additives for ruminant production and sustainability

    The global demand for animal-derived foods has led to a substantial expansion in ruminant production, which has raised concerns regarding methane emissions. To address these challenges, microalgal species that...

    Mianmian Zhu, Stacy D. Singer, Le Luo Guan, Guanqun Chen in Advanced Biotechnology (2024)

  2. No Access

    Protocol

    Eliciting Targeted Mutations in Medicago sativa Using CRISPR/Cas9-Mediated Genome Editing: A Potential Tool for the Improvement of Disease Resistance

    CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9) has become a breeding tool of choice for eliciting targeted genetic alterations in crop species as a means of...

    Udaya Subedi, Kimberley Burton Hughes, Guanqun Chen in Plant-Pathogen Interactions (2023)

  3. No Access

    Article

    CW198 acts as a genetic insulator to block enhancer-promoter interaction in plants

    Insulators in vertebrates play a role in genome architecture and orchestrate temporo-spatial enhancer-promoter interactions. In plants, insulators and their associated binding factors have not been documented ...

    Li Jiang, Yue Liu, Zhifeng Wen, Yingjun Yang, Stacy D. Singer in Transgenic Research (2022)

  4. No Access

    Article

    The effect of AINTEGUMENTA-LIKE 7 over-expression on seed fatty acid biosynthesis, storage oil accumulation and the transcriptome in Arabidopsis thaliana

    AIL7 over-expression modulates fatty acid biosynthesis and triacylglycerol accumulation in Arabidopsis develo** seeds through the transcriptional regulation of associated genes.

    Stacy D. Singer, Kethmi N. Jayawardhane, Chen Jiao in Plant Cell Reports (2021)

  5. No Access

    Article

    Seed-specific down-regulation of Arabidopsis CELLULOSE SYNTHASE 1 or 9 reduces seed cellulose content and differentially affects carbon partitioning

    Seed-specific down-regulation of AtCESA1 and AtCESA9, which encode cellulose synthase subunits, differentially affects seed storage compound accumulation in Arabidopsis.

    Kethmi N. Jayawardhane, Stacy D. Singer, Jocelyn A. Ozga in Plant Cell Reports (2020)

  6. No Access

    Article

    Castor patatin-like phospholipase A IIIβ facilitates removal of hydroxy fatty acids from phosphatidylcholine in transgenic Arabidopsis seeds

    Castor patatin-like phospholipase A IIIβ facilitates the exclusion of hydroxy fatty acids from phosphatidylcholine in develo** transgenic Arabidopsis seeds.

    Yingyu Lin, Guanqun Chen, Elzbieta Mietkiewska, Ziliang Song in Plant Molecular Biology (2019)

  7. No Access

    Article

    Biotechnological strategies for improved photosynthesis in a future of elevated atmospheric CO2

    The improvement of photosynthesis using biotechnological approaches has been the focus of much research. It is now vital that these strategies be assessed under future atmospheric conditions.

    Stacy D. Singer, Raju Y. Soolanayakanahally, Nora A. Foroud, Roland Kroebel in Planta (2019)

  8. No Access

    Article

    Bioactivity and biotechnological production of punicic acid

    Punicic acid (PuA; 18: 3Δ 9cis,11trans,13cis ) is an unusual 18-carbon fatty acid bearing three conjugated double bonds. It has been shown to exhibit a myriad of beneficial bioac...

    Roman Holic, Yang Xu, Kristian Mark P. Caldo in Applied Microbiology and Biotechnology (2018)

  9. No Access

    Book

  10. No Access

    Chapter

    Building a Case for Plant Bioproducts

    Global carbon emissions associated with fossil fuel dependence are increasing steadily and are a major root cause of climate change. In order to reduce our reliance on fossil fuels, there is an imminent need t...

    Randall J. Weselake, Guanqun Chen, Stacy D. Singer in Plant Bioproducts (2018)

  11. No Access

    Chapter

    Introduction to Plant Biomolecules and Cellular Metabolism

    Lipids, carbohydrates, and proteins represent the major starting materials for the production of various bioproducts in plants. These organic compounds contain carbon atoms that share electrons with hydrogen a...

    Randall J. Weselake, Stacy D. Singer, Guanqun Chen in Plant Bioproducts (2018)

  12. No Access

    Chapter

    An Integrated Approach to Plant Bioproduct Production

    The area of plant bioproducts comprises several interdependent and allied disciplines, including plant biochemistry and physiology, plant breeding, agronomy, engineering, biotechnology, biorefining, and the so...

    Randall J. Weselake, Stacy D. Singer, Guanqun Chen in Plant Bioproducts (2018)

  13. No Access

    Chapter

    Production of Other Bioproducts from Plant Oils

    Plant oils have many additional applications other than biofuel. Potential industrial applications of plant lipids include the production of lubricants, solvents, surfactants, bioplastics, and rubber. There ha...

    Stacy D. Singer, Randall J. Weselake in Plant Bioproducts (2018)

  14. No Access

    Chapter

    Biomass-Derived Building Block Chemicals

    The chemical industry is currently based on simple molecules derived from petroleum. These simple molecules, called building block chemicals, can also be produced from plant biomass. Building block chemicals c...

    Lucas J. Falarz, Stacy D. Singer, Guanqun Chen in Plant Bioproducts (2018)

  15. No Access

    Chapter

    Production of Biodiesel from Plant Oils

    Biodiesel is composed of alkyl esters of vegetable oil or animal fat and is produced primarily through a process known as transesterification. The functional properties of biodiesel are determined by the compo...

    Stacy D. Singer, Randall J. Weselake in Plant Bioproducts (2018)

  16. Article

    Open Access

    Heterologous expression of flax PHOSPHOLIPID:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE (PDCT) increases polyunsaturated fatty acid content in yeast and Arabidopsis seeds

    Flax (Linum usitatissimum L.) is an agriculturally important crop with seed oil enriched in α-linolenic acid (18:3 cisΔ9, 12, 15; ALA). This polyunsaturated fatty acid (PUFA) is the major det...

    Aruna D Wickramarathna, Rodrigo M P Siloto, Elzbieta Mietkiewska in BMC Biotechnology (2015)

  17. Article

    Open Access

    Gibberellin-induced changes in the transcriptome of grapevine (Vitis labrusca × V. vinifera) cv. Kyoho flowers

    Gibberellins are well known for their growth control function in flower, fruit and seed development, and as such, exogenous gibberellic acid (GA) application plays an important role in viticulture. Unfortunate...

    Chenxia Cheng, Chen Jiao, Stacy D Singer, Min Gao, **aozhao Xu, Yiming Zhou in BMC Genomics (2015)

  18. No Access

    Article

    The grape VvMBF1 gene improves drought stress tolerance in transgenic Arabidopsis thaliana

    Multiprotein bridging factor 1 (MBF1) is an evolutionarily conserved transcriptional co-activator in archaea and eukaryotes that has been demonstrated previously to play an important role in various types of ...

    Qin Yan, Hongmin Hou, Stacy D. Singer in Plant Cell, Tissue and Organ Culture (PCTO… (2014)

  19. Article

    Open Access

    Development and characterization of low α-linolenic acid Brassica oleracea lines bearing a novel mutation in a ‘class a’ FATTY ACID DESATURASE 3gene

    Traditional canola (Brassica napus L.; AACC, 2n = 38) cultivars yield seed oil with a relatively high proportion of α-linolenic acid (ALA; C18:3cisΔ9,12,15), which is desirable from a health perspective. Unfortun...

    Stacy D Singer, Randall J Weselake, Habibur Rahman in BMC Genetics (2014)

  20. No Access

    Article

    Two tobacco AP1-like gene promoters drive highly specific, tightly regulated and unique expression patterns during floral transition, initiation and development

    The genetic engineering of agronomic traits requires an array of highly specific and tightly regulated promoters that drive expression in floral tissues. In this study, we isolated and characterized two tobacco A...

    **** Zhang, Guohua Yan, Zhifeng Wen, Young-Qiang An, Stacy D. Singer in Planta (2014)

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