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Article
Open AccessZeb2 regulates differentiation of long-lived effector of invariant natural killer T cells
After activation, some invariant natural killer T (iNKT) cells are differentiated into Klrg1+ long-lived effector NKT1 cells. However, the regulation from the effector phase to the memory phase has not been eluci...
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Article
Open AccessAssociation of cellular immunity with severity of COVID-19 from the perspective of antigen-specific memory T cell responses and cross-reactivity
Coronaviruses regularly cause outbreaks of zoonotic diseases characterized by severe pneumonia. The new coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has caused the global pandemic...
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Article
Open AccessIdentification of TCR repertoires in functionally competent cytotoxic T cells cross-reactive to SARS-CoV-2
SARS-CoV-2-specific CD8+ T cells are scarce but detectable in unexposed healthy donors (UHDs). It remains unclear whether pre-existing human coronavirus (HCoV)-specific CD8+ T cells are converted to functionally ...
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Article
Open AccessInfluenza virus infection expands the breadth of antibody responses through IL-4 signalling in B cells
Influenza viruses are a major public health problem. Vaccines are the best available countermeasure to induce effective immunity against infection with seasonal influenza viruses; however, the breadth of antib...
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Article
Multiple-channel Concentric Grid Nebulizer for Online Standard Addition in Inductively Coupled Plasma Optical Emission Spectrometry
A multiple channel-type concentric grid nebulizer (m-CGrid) was developed for realizing efficient online standard addition in inductively coupled plasma optical emission spectrometry (ICP-OES) without premixin...
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Article
Open AccessRunx-mediated regulation of CCL5 via antagonizing two enhancers influences immune cell function and anti-tumor immunity
CCL5 is a unique chemokine with distinct stage and cell-type specificities for regulating inflammation, but how these specificities are achieved and how CCL5 modulates immune responses is not well understood. ...
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Article
Development of certified reference material NMIJ CRM 6205-a for the validation of DNA quantification methods: accurate mass concentrations of 600-bp DNA solutions having artificial sequences
Two 600-bp DNA solutions (DNA600-G and DNA600-T) were developed as certified reference material, NMIJ CRM 6205-a, for the validation of DNA quantification methods. Both DNA600-G and DNA600-T are ideal as “spik...
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Article
Open AccessEomes transcription factor is required for the development and differentiation of invariant NKT cells
Eomes regulates the differentiation of CD8+ T cells into effector and memory phases. However, its role in invariant (i)NKT cells remains unknown. Here, we show the impact of Eomes on iNKT cells in the thymus and ...
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Article
Open AccessRNA-transfection of γ/δ T cells with a chimeric antigen receptor or an α/β T-cell receptor: a safer alternative to genetically engineered α/β T cells for the immunotherapy of melanoma
Adoptive T-cell therapy relying on conventional T cells transduced with T-cell receptors (TCRs) or chimeric antigen receptors (CARs) has caused substantial tumor regression in several clinical trials. However,...
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Article
High transport efficiency of nanoparticles through a total-consumption sample introduction system and its beneficial application for particle size evaluation in single-particle ICP-MS
In order to facilitate reliable and efficient determination of both the particle number concentration (PNC) and the size of nanoparticles (NPs) by single-particle ICP-MS (spICP-MS) without the need to correct ...
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Chapter
Single Cell Analysis by Using ICP-MS
Single cell analysis by using inductively coupled plasma mass spectrometry (ICP-MS) has been attracting attention for investigating a cell-to-cell variation of content of elements and biomolecules. ICP-MS has ...
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Article
Open AccessIn vivo dendritic cell targeting cellular vaccine induces CD4+ Tfh cell-dependent antibody against influenza virus
An induction of long-term cellular and humoral immunity is for the goal of vaccines, but the combination of antigens and adjuvant remain unclear. Here, we show, using a cellular vaccine carrying foreign protei...
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Article
Open AccessEAF2 mediates germinal centre B-cell apoptosis to suppress excessive immune responses and prevent autoimmunity
Regulated apoptosis of germinal centre (GC) B cells is critical for normal humoral immune responses. ELL-associated factor 2 (EAF2) regulates transcription elongation and has been shown to be an androgen-respo...
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Article
Development of C-reactive protein certified reference material NMIJ CRM 6201-b: optimization of a hydrolysis process to improve the accuracy of amino acid analysis
To standardize C-reactive protein (CRP) assays, the National Metrology Institute of Japan (NMIJ) has developed a C-reactive protein solution certified reference material, CRM 6201-b, which is intended for use ...
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Article
HPLC for Separation and Quantification of Deoxyribonucleic Acid Fragments and Measurement of Deoxyribonucleic Acid Degradation
DNA quantification has made its mark in pharmaceutical analysis and the life sciences. In particular, in the quality control of nucleic acid drugs and the detection and quantification of genetically modified o...
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Article
Time-resolved ICP-MS Measurement: a New Method for Elemental and Multiparametric Analysis of Single Cells
Time-resolved inductively coupled plasma mass spectrometry (ICP-MS) has attracted much attention for elemental and multiparametric analysis of single cells, instead of a classical bulk analysis of large amount...
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Article
High Sensitive Elemental Analysis of Single Yeast Cells (Saccharomyces cerevisiae) by Time-Resolved Inductively-Coupled Plasma Mass Spectrometry Using a High Efficiency Cell Introduction System
Trace elemental analysis of single yeast cells with time-resolved inductively coupled plasma mass spectrometry (ICP-MS) was successfully carried out, where a high efficiency cell introduction system (HECIS) co...
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Article
Accumulation of Activated Invariant Natural Killer T Cells in the Tumor Microenvironment after α-Galactosylceramide-Pulsed Antigen Presenting Cells
The intravenous administration of α-Galactosylceramide (α-GalCer)-pulsed antigen presenting cells (APCs) is well tolerated and the increased IFN-γ producing cells in the peripheral blood after the treatment ap...
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Article
Induced pluripotency as a potential path towards iNKT cell-mediated cancer immunotherapy
Invariant natural killer T (iNKT) cells are characterized by the expression of an invariant Vα14–Jα18 paired with Vβ8/7/2 in mice, and Vα24–Jα18 with Vβ11 in humans, that recognizes glycolipids, such as α-galacto...
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Chapter
Introduction: Mechanisms of NKT-Cell-Mediated Adjuvant Activity and Function of iPS-Derived NKT Cells
Natural killer T (NKT) cells are characterized by the expression of an invariant Vα14Jα18 receptor that recognizes glycolipids such as α-galactosylceramide (α-GalCer) in conjunction with CD1d molecule. Functio...
