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Article
Open AccessRaman image-activated cell sorting
The advent of image-activated cell sorting and imaging-based cell picking has advanced our knowledge and exploitation of biological systems in the last decade. Unfortunately, they generally rely on fluorescent...
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Article
Open AccessVirtual-freezing fluorescence imaging flow cytometry
By virtue of the combined merits of flow cytometry and fluorescence microscopy, imaging flow cytometry (IFC) has become an established tool for cell analysis in diverse biomedical fields such as cancer biology...
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Article
Open AccessMast cells play role in wound healing through the ZnT2/GPR39/IL-6 axis
Zinc (Zn) is an essential nutrient and its deficiency causes immunodeficiency and skin disorders. Various cells including mast cells release Zn-containing granules when activated; however, the biological role ...
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Article
Open AccessA Novel Mouse Model of iNKT Cell-deficiency Generated by CRISPR/Cas9 Reveals a Pathogenic Role of iNKT Cells in Metabolic Disease
iNKT cells play important roles in immune regulation by bridging the innate and acquired immune systems. The functions of iNKT cells have been investigated in mice lacking the Traj18 gene segment that were genera...
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Article
Probing the metabolic heterogeneity of live Euglena gracilis with stimulated Raman scattering microscopy
Understanding metabolism in live microalgae is crucial for efficient biomaterial engineering, but conventional methods fail to evaluate heterogeneous populations of motile microalgae due to the labelling requi...
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Chapter
Elucidation and Control of the Mechanisms Underlying Chronic Inflammation Mediated by Invariant Natural Killer T Cells
Invariant natural killer T (iNKT) cells are a subpopulation of T lymphocytes with unique specificities against (glyco)lipids presented in the context of CD1d belonging to major histocompatibility complex (MHC)...
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Article
Induced pluripotency as a potential path towards iNKT cell-mediated cancer immunotherapy
Invariant natural killer T (iNKT) cells are characterized by the expression of an invariant Vα14–Jα18 paired with Vβ8/7/2 in mice, and Vα24–Jα18 with Vβ11 in humans, that recognizes glycolipids, such as α-galacto...
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Chapter
Introduction: Mechanisms of NKT-Cell-Mediated Adjuvant Activity and Function of iPS-Derived NKT Cells
Natural killer T (NKT) cells are characterized by the expression of an invariant Vα14Jα18 receptor that recognizes glycolipids such as α-galactosylceramide (α-GalCer) in conjunction with CD1d molecule. Functio...
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Article
The transcription factor E4BP4 regulates the production of IL-10 and IL-13 in CD4+ T cells
Chronic stimulation of lymphocytes results in upregulation of immunomodulatory IL-10, but the molecular mechanisms of this process have remained unknown. Kubo and colleagues demonstrate that the transcription ...
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Article
A sodium hyaluronate carboxymethylcellulose bioresorbable membrane prevents postoperative small-bowel adhesive obstruction after distal gastrectomy
It is predictable that since distal gastrectomy (DG) with Billroth I anastomosis involves no procedures caudal to transverse colon, the effects of the surgical wound are the main cause of adhesive obstruction....
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Article
Regulation of IL-9 expression by IL-25 signaling
How IL-9 expression is regulated remains unclear. Dong and co-workers show that the IL-25–IL-17RB pathway is central to the physiological regulation of this cytokine in vivo.
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Article
M-Sec promotes membrane nanotube formation by interacting with Ral and the exocyst complex
How tunnelling nanotubes form between cells is unclear. A mammalian protein, M-Sec, that has homology to the Sec6 subunit of the exocyst complex, is sufficient and necessary for nanotube formation. The Ral GTP...
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Article
Assessment of pain by face scales after gastrectomy: comparison of laparoscopically assisted gastrectomy and open gastrectomy
Laparoscopic gastrectomy is reported to cause little pain. However, only the total number of analgesics used has been studied to date. Because pain is a subjective experience, its evaluation requires indicator...
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Article
Inspection of Safety and Accuracy of D2 Lymph Node Dissection in Laparoscopy-Assisted Distal Gastrectomy
There is a consensus on the indication of laparoscopy-assisted distal gastrectomy (LADG) for early gastric cancer that needs D1 + α or D1 + β lymph node dissection. However, many gastrointestinal surgeons cons...
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Article
Methods for detection, isolation and culture of mouse and human invariant NKT cells
This protocol describes methods to identify, purify and culture CD1d restricted invariant natural killer T (iNKT) cells from mouse tissue or human blood samples. The methods for identification and purification of...
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Article
Classification for Dimethylarsenate-decomposing Bacteria Using a Restrict Fragment Length Polymorphism Analysis of 16S rRNA Genes
A new monitoring system for bacterial communities involving dimethylarsinic acid (DMAA) decomposition was provided by combining the MPN (Most Probable Number) method and RFLP (restriction-fragment-length polym...
