10 Result(s)
within
Seong Hee Bhoo
Chemistry
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Article
Open AccessBiochemical characterization of a regiospecific flavonoid 3'-O-methyltransferase from orange
Citrus plants have diverse methoxyflavonoids including, chrysoeriol, isosakuranetin, and nobiletin. In plants, O-methyltransferases (OMTs) participate in the methylation of a vast array of secondary metabolites, ...
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Article
Immuno-affinity purification of 2B8-tagged proteins
Detection, purification and characterization of proteins are essential procedures in the field of biochemistry. Epitope tag systems are commonly used to characterize unknown proteins. The Deinococcus radiodurans ...
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Article
Characterization of Arabidopsis mutants insensitive to high sugar concentrations
Sugar is appreciated to function as a signaling molecule by controlling gene expression, thereby regulating various developmental processes in plants. We screened sucrose-insensitive (sin) mutants in Arabidopsis ...
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Article
An efficient method for detection of recombinant proteins using a bacteriophytochrome chromophore binding domain from Deinococcus Radiodurans
Bacteriophytochromes are composed of N-terminal region for chromophore binding and C-terminal domain for transmission of a light signal. In this study, the possibility of using the chromophore-binding domain f...
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Article
Characterization of regiospecific flavonoid 3′/5′-O-methyltransferase from tomato and its application in flavonoid biotransformation
Flavonoids are ubiquitous secondary metabolites in plants and possess a wide range of biological activities such as anti-oxidant, anti-microbial, and anti-cancer activities. As a food ingredient, flavonoids ar...
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Article
Molecular characterization of phytochromeinteracting nascent polypeptide associated complex protein in Arabidopsis thaliana
Nascent polypeptide associated complex (NAC) proteins bind to newly synthesized polypeptide chains from ribosome and are involved in various physiological processes. An in vitro pulldown assay demonstrated that t...
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Article
Purification and Characterization of a Recombinant Bacteriophytochrome of Xanthomonas oryzae pathovar oryzae
Phytochrome-like proteins have been recently identified in prokaryotes but their features and functions are not clear. We cloned a gene encoding the phytochrome-like protein (XoBphP) in a pathogenic bacteria, Xan...
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Article
Rice protein disulfide isomerase (OsPDI) and rice chaperone binding protein (OsBiP) levels are not directly correlated with the eating quality of rice
The composition and distribution of seed storage proteins are important factors for eating quality such as flavor and texture in rice. The levels of rice protein disulfide isomerase and rice chaperone binding ...
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Article
LAF1 positively regulates PhyA signaling by modulation of COP1 activity
It was known that long after far-red light 1 (LAF1), a positive phytochrome A (phyA) signal transducer, is ubiquitinated by constitutive photomorphogenic 1 (COP1) in vitro and stabilizes long hypocotyl in far-red...
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Article
Differential interactions of phytochrome A (Pr vs. Pfr) with monoclonal antibodies probed by a surface plasmon resonance technique
Phytochromes are red- and far-red light-reversible photoreceptors for photomorphogenesis in plants. Phytochrome A is a dimeric chromopeptide that mediates very low fluence and high irradiance responses. To ana...
