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Article
Open AccessBiochemical characterization of a regiospecific flavonoid 3'-O-methyltransferase from orange
Citrus plants have diverse methoxyflavonoids including, chrysoeriol, isosakuranetin, and nobiletin. In plants, O-methyltransferases (OMTs) participate in the methylation of a vast array of secondary metabolites, ...
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Article
Open AccessAntibody production and characterization of the nucleoprotein of sever fever with thrombocytopenia syndrome virus (SFTSV) for effective diagnosis of SFTSV
Severe fever with thrombocytopenia syndrome (SFTS) is an infectious disease caused by the Dabie bandavirus, [or SFTS virus (SFTSV)] that has become increasingly widespread since it was first reported in 2009. The...
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Article
Immuno-affinity purification of 2B8-tagged proteins
Detection, purification and characterization of proteins are essential procedures in the field of biochemistry. Epitope tag systems are commonly used to characterize unknown proteins. The Deinococcus radiodurans ...
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Article
Characterization of Arabidopsis mutants insensitive to high sugar concentrations
Sugar is appreciated to function as a signaling molecule by controlling gene expression, thereby regulating various developmental processes in plants. We screened sucrose-insensitive (sin) mutants in Arabidopsis ...
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Article
Pyrophosphate: fructose-6-phosphate 1-phosphotransferase is involved in the tolerance of Arabidopsis seedlings to salt and osmotic stresses
In plants, pyrophosphate: fructose-6-phosphate 1-phosphotransferase (PFP) is a regulatory enzyme that participates in glycolysis and gluconeogenesis. Arabidopsis contains two PFPα subunit genes (PFPα1 and PFPα2) ...
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Article
Proteome analysis of chlorotic leaves of the Arabidopsis mex1 mutant defective in the mobilization of starch degradation products
Leaf starch synthesized during the day for transient storage of photoassimilated carbon is degraded the following night to support respiration and growth in plants. Maltose is a major product of starch degrada...
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Article
Rapid transient expression of cholera toxin B subunit (CTB) in Nicotiana benthamiana
Cholera toxin B subunit (CTB) has the potential to be an effective adjuvant for mucosal vaccines because of its ability to increase antigen uptake and presentation by antigen-presenting cells through GM1-gangl...
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Article
An efficient method for detection of recombinant proteins using a bacteriophytochrome chromophore binding domain from Deinococcus Radiodurans
Bacteriophytochromes are composed of N-terminal region for chromophore binding and C-terminal domain for transmission of a light signal. In this study, the possibility of using the chromophore-binding domain f...
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Article
Characterization of regiospecific flavonoid 3′/5′-O-methyltransferase from tomato and its application in flavonoid biotransformation
Flavonoids are ubiquitous secondary metabolites in plants and possess a wide range of biological activities such as anti-oxidant, anti-microbial, and anti-cancer activities. As a food ingredient, flavonoids ar...
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Article
Molecular characterization of phytochromeinteracting nascent polypeptide associated complex protein in Arabidopsis thaliana
Nascent polypeptide associated complex (NAC) proteins bind to newly synthesized polypeptide chains from ribosome and are involved in various physiological processes. An in vitro pulldown assay demonstrated that t...
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Article
Manipulation of triose phosphate/phosphate translocator and cytosolic fructose-1,6-bisphosphatase, the key components in photosynthetic sucrose synthesis, enhances the source capacity of transgenic Arabidopsis plants
Photoassimilated carbons are converted to sucrose in green plant leaves and distributed to non-phototropic tissues to provide carbon and energy. In photosynthetic sucrose biosynthesis, the chloroplast envelope...
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Article
Purification and Characterization of a Recombinant Bacteriophytochrome of Xanthomonas oryzae pathovar oryzae
Phytochrome-like proteins have been recently identified in prokaryotes but their features and functions are not clear. We cloned a gene encoding the phytochrome-like protein (XoBphP) in a pathogenic bacteria, Xan...
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Article
The ethylene signaling pathway has a negative impact on sucrose-induced anthocyanin accumulation in Arabidopsis
In an attempt to understand the complex regulatory mechanisms underlying sucrose-induced flavonoid biosynthesis, we examined several Arabidopsis mutants with altered anthocyanin accumulation. We determined tha...
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Article
Rice protein disulfide isomerase (OsPDI) and rice chaperone binding protein (OsBiP) levels are not directly correlated with the eating quality of rice
The composition and distribution of seed storage proteins are important factors for eating quality such as flavor and texture in rice. The levels of rice protein disulfide isomerase and rice chaperone binding ...
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Article
Improvement of plant protein solubilization and 2-DE gel resolution through optimization of the concentration of Tris in the solubilization buffer
It is important to solubilize acetone-precipitated proteins before isoelectric focusing (IEF) to achieve high resolution 2-DE gels. To resolve the maximum possible number of plant protein spots, we developed a...
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Article
LAF1 positively regulates PhyA signaling by modulation of COP1 activity
It was known that long after far-red light 1 (LAF1), a positive phytochrome A (phyA) signal transducer, is ubiquitinated by constitutive photomorphogenic 1 (COP1) in vitro and stabilizes long hypocotyl in far-red...
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Article
Characterization of Arabidopsis RopGEF family genes in response to abiotic stresses
Rho-related GTPase of plants (ROP) plays an important role in plant growth and development as a signaling protein. Plant RopGEFs are recently identified ROP activator proteins in Arabidopsis. In this study, we cl...
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Article
Altered expression of pyrophosphate: Fructose-6-phosphate 1-phosphotransferase affects the growth of transgenic Arabidopsis plants
Pyrophosphate: fructose-6-phosphate 1-phosphotransferase (PFP) catalyzes the reversible interconversion of fructose-6-phosphate and fructose-1,6-bisphosphate, a key step in the regulation of the metabolic flux...
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Article
The effect of DTT in protein preparations for proteomic analysis: Removal of a highly abundant plant enzyme, ribulose bisphosphate carboxylase/oxygenase
Rubisco is a major photosynthetic plant enzyme in the chloroplasts, catalyzing a photosynthetic reaction through carboxylation and oxygenation in the leaves. Despite its biological importance, its high abundan...
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Article
Identification of the ADP-glucose pyrophosphorylase isoforms essential for starch synthesis in the leaf and seed endosperm of rice (Oryza sativa L.)
ADP-glucose pyrophosphorylase (AGP) catalyzes the first committed step of starch biosynthesis in higher plants. To identify AGP isoforms essential for this biosynthetic process in sink and source tissues of ri...
