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Article
Functional divergence of two closely related carboxylesterases in pepper (Capsicum annuum L.)
Carboxylesterases (CXEs) catalyze the hydrolysis of carboxylic acid esters into the corresponding acids and alcohols. Previously, a carboxylesterase gene was isolated from pepper (Capsicum annuum L.) and designat...
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Article
CRISPR/Cas9-mediated mutation of 5-oxoprolinase gene confers resistance to sulfonamide compounds in Arabidopsis
Weeds are important biotic constraints to agricultural production, so herbicides are widely used with agronomic crops as the primary method of weed control. Accordingly, extensive efforts to develop herbicide-...
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Article
Overexpression of Arabidopsis thaliana blue-light inhibitor of cryptochromes 1 gene alters plant architecture in soybean
Arabidopsis thaliana blue-light inhibitor of cryptochromes 1 (AtBIC1) suppresses the dimerization of cryptochromes, which is necessary for cryptochrome function in plants. Additionally, AtBIC1 interacts with bras...
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Article
Generation and Characterization of a Specific Polyclonal Antibody against Arabidopsis thaliana Phytochrome-Interacting Factor 3
Phytochrome-interacting factors (PIFs) are a basic helix-loop-helix family of transcriptional regulators that maintain skotomorphogenesis and suppress photomorphogenesis. PIFs are regulated by plant photorecep...
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Article
Advanced strategies to control plant pathogenic fungi by host-induced gene silencing (HIGS) and spray-induced gene silencing (SIGS)
Plant pathogenic fungi and oomycetes cause severe losses of crop yield worldwide. Fungicides are widely applied to manage plant diseases caused by pathogenic fungi, but fungicide-resistant fungal populations h...
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Article
Plant Thermomorphogenic Adaptation to Global Warming
Plants are capable of actively adapting to a variety of environmental signals by adjusting physiological activities, growth behaviors, and architectural morphology. Temperatures are one of the major environmen...
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Article
New era of precision plant breeding using genome editing
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Article
Application of CRISPR/Cas9-mediated gene editing for the development of herbicide-resistant plants
Herbicide resistance is one of the most important traits for plant biotechnology, which is widely used to improve agricultural efficiency by controlling weeds and also used as a selectable marker during geneti...
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Article
Open AccessA phyB-PIF1-SPA1 kinase regulatory complex promotes photomorphogenesis in Arabidopsis
CONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1) is a highly conserved E3 ubiquitin ligase from plants to animals and acts as a central repressor of photomorphogenesis in plants. SUPPRESSOR OF PHYA-105 1 family members...
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Protocol
Expression, Purification, and Spectral Characterization of Phytochromes
Expression and purification of recombinant proteins are important for the structure–function study of phytochromes. However, it is difficult to purify phytochrome proteins from natural sources or using a bacte...
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Article
Root-specific expression of defensin in transgenic tobacco results in enhanced resistance against Phytophthora parasitica var. nicotianae
Phytophthora species are soil-borne pathogens that damage plants in both agro- and natural ecosystems. To suppress the devastating pathogen, we generated a root-specific expression system using a specific promote...
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Article
Constitutive expression of a fungus-inducible carboxylesterase improves disease resistance in transgenic pepper plants
Resistance against anthracnose fungi was enhanced in transgenic pepper plants that accumulated high levels of a carboxylesterase, PepEST in anthracnose-susceptible fruits, with a concurrent ind...
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Article
Open AccessEvidence that phytochrome functions as a protein kinase in plant light signalling
It has been suggested that plant phytochromes are autophosphorylating serine/threonine kinases. However, the biochemical properties and functional roles of putative phytochrome kinase activity in plant light s...
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Article
Transgenic Turfgrasses Expressing Hyperactive Ser599Ala Phytochrome A Mutant Exhibit Abiotic Stress Tolerance
Turfgrasses are environmentally and recreationally valuable plants that are constantly subjected to various forms of stress in their artificial and natural habitats. Previously, it was shown that the transform...
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Article
Characterization of a small constitutive promoter from Arabidopsis translationally controlled tumor protein (AtTCTP) gene for plant transformation
A plant-derived 0.3 kb constitutive promoter was obtained from AtTCTP expression analysis, and successfully applied to the expression of a selectable marker ...
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Article
Cellular localization and detergent dependent oligomerization of rice allene oxide synthase-1
Allene oxide synthase-1 from Oryza sativa (OsAOS1) localizes to the chloroplast, but lacks a putative chloroplast targeting sequence typically found in dicot AOS. Here, kinetic parameters and the oligomerization ...
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Article
Functional characterization of a chloroplast-targeted RNA-binding protein CRP1 in Arabidopsis thaliana under abiotic stress conditions
Although recent analysis of proteomes in specific cellular organelles of plants has demonstrated that more than 2,000 nuclear-encoded proteins are targeted to the chloroplast, the physiological functions of a ...
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Article
Open AccessAgrobacterium-mediated genetic transformation of Miscanthus sinensis
Miscanthus species are tall perennial rhizomatous grasses with C4 photosynthesis originating from East Asia, and they are considered as important bioenergy crops for biomass production. I...
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Article
Overexpression of Arabidopsis ABF3 gene confers enhanced tolerance to drought and heat stress in cree** bentgrass
The Arabidopsis, abscisic acid responsive element-binding factor 3, ABF3 is known to play an important role in stress responses via regulating the expression of stress-responsive genes. In this study, we introduc...
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Article
Erratum to: Overexpression of phytochrome A and its hyperactive mutant improves shade tolerance and turf quality in cree** bentgrass and zoysiagrass
