5 Result(s)
within
Sebastian M Fica
Science, Humanities and Social Sciences, multidisciplinary
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Article
Open AccessMonovalent metal ion binding promotes the first transesterification reaction in the spliceosome
Cleavage and formation of phosphodiester bonds in nucleic acids is accomplished by large cellular machineries composed of both protein and RNA. Long thought to rely on a two-metal-ion mechanism for catalysis, ...
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Article
Open AccesspsiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation
RNA helicases remodel the spliceosome to enable pre-mRNA splicing, but their binding and mechanism of action remain poorly understood. To define helicase-RNA contacts in specific spliceosomal states, we develo...
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Article
Structure of a spliceosome remodelled for exon ligation
The cryo-electron microscopy structure of a yeast spliceosome stalled before mature RNA formation provides insight into the mechanism of exon ligation.
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Article
Cryo-EM structure of the spliceosome immediately after branching
Precursor mRNA (pre-mRNA) splicing proceeds by two consecutive transesterification reactions via a lariat–intron intermediate. Here we present the 3.8 Å cryo-electron microscopy structure of the spliceosome im...
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Article
RNA catalyses nuclear pre-mRNA splicing
In nuclear pre-messenger RNA splicing, introns are excised by the spliceosome, a dynamic machine composed of both proteins and small nuclear RNAs (snRNAs). Over thirty years ago, after the discovery of self-sp...
