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Article
Gene Expression Profiling as a New Real-Time Assay in Human Biomonitoring of Waste-to-Energy Plant Workers
Exposure to heavy metals represents one of the most important risk factors for the health of incinerator workers. Indeed, heavy metals can determine increased generation of reactive oxygen species (ROS). In th...
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Protocol
Untargeted Metabolomics of Plant Leaf Tissues
Untargeted metabolomics is a useful approach for the simultaneous analysis of a vast array of compounds from a single extract. Metabolomic profiling is the relative multi-parallel quantification of a mixture o...
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Article
Open AccessMetabolomics and proteomics reveal drought-stress responses of leaf tissues from spring-wheat
To reveal the integrative biochemical networks of wheat leaves in response to water deficient conditions, proteomics and metabolomics were applied to two spring-wheat cultivars (Bahar, drought-susceptible; Kavir,...
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Chapter
What Can Small Molecules Tell Us About Cold Stress Tolerance in Plants?
Plants display a wide capacity range to survive cold and freezing conditions. Indeed, they are able to sense low, non-freezing temperatures and activate processes that lead to an increase in freezing tolerance...
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Article
Open AccessCDK1 phosphorylates WRN at collapsed replication forks
Regulation of end-processing is critical for accurate repair and to switch between homologous recombination (HR) and non-homologous end joining (NHEJ). End resection is a two-stage process but very little is k...
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Chapter and Conference Paper
Untargeted metabolomic analyses open new scenarios in post mortem pig muscles: Casertana and Large White
Post mortem metabolism of pig muscles represents a pivotal area of research, in that pig meat quality is largely influenced by biochemical changes arising soon after slaughter. Metabolic rate and, ...
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Article
Changes in morphology, cell wall composition and soluble proteome in Rhodobacter sphaeroides cells exposed to chromate
The response of the carotenoidless Rhodobacter sphaeroides mutant R26 to chromate stress under photosynthetic conditions is investigated by biochemical and spectroscopic measurements, proteomic analysis and cell ...
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Protocol
Combinatorial Peptide Ligand Libraries to Discover Liver Disease Biomarkers in Plasma Samples
High-abundance proteins present in blood plasma make the detection of low-abundance proteins extremely difficult by proteomics technology. Hexapeptide combinatorial ligand libraries can be used to investigate ...
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Article
Depletion of hemoglobin and carbonic anhydrase from erythrocyte cytosolic samples by preparative clear native electrophoresis
Proteomic analysis of red cells is compromised by the presence of high-abundance proteins (hemoglobin and carbonic anhydrase-1), which completely obscure low-abundance species. The depletion method presented h...
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Chapter and Conference Paper
Effect of Cobalt Ions on the Soluble Proteome of a Rhodobacter sphaeroides Carotenoidless Mutant
Rhodobacter sphaeroides strain R26.1 showed tolerance to Co2+ ions, up to 10 mM concentration. Interestingly the bacteriochlorophyll biosynthesis was found to decrease upon addition of such metal i...