Enzyme Immunoassays
From Concept to Product Development
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Synthesis, characterization and photocatalytic behavior of TiO2–SiO2 mesoporous composites in hydrogen generation from water splitting
TiO2–SiO2 mesoporous composite photocatalysts with different proportions (in wt%) of TiO2 and SiO2 (TiO2–SiO2 = 20:80, 40:60, 60:40, 80:20 and 100:0) were prepared by loading TiO2 on as-synthesized Si–MCM-41 usin...
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Role of do**-induced photochemical and microstructural properties in the photocatalytic activity of InVO4 for splitting of water
We report in this paper on microstructural, optical and photocatalytic properties of single-phase indium orthovanadates, as a function of do** at lattice sites. The UV–visible spectra of these samples exhibi...
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Synthesis of N-methylaniline by Aniline Alkylation with Methanol over Sn-MFI Molecular Sieve
Sn-MFI molecular sieves (Tin silicalite-1) with SiO2/SnO2 molar ratio of 50–150 were synthesized and characterized. Elemental analysis, XRD and DRUV–vis confirmed that prepared samples were of MFI zeolite structu...
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Article
Vitamin B12 and homocysteine status in asymptomatic Indian toddlers
Low serum vitamin B12 (V B12) and hyperhomocysteinemia have been reported in asymptomatic Asian Indian men. We studied the prevalence of V B12 deficiency and hyperhomocysteinemia in 51 asymptomatic toddlers, from...
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Open AccessVitamin B12 and folate concentrations during pregnancy and insulin resistance in the offspring: the Pune Maternal Nutrition Study
Raised maternal plasma total homocysteine (tHcy) concentrations predict small size at birth, which is a risk factor for type 2 diabetes mellitus. We studied the association between maternal vitamin B12, folate an...
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Heterogenized Molybdovanadophosphoric Acid on Amine-Functionalized SBA-15 for Selective Oxidation of Alkenes
Mono-vanadium substituted molybdovanadophosphoric acid has been immobilized on amine-functionalized SBA-15 and characterized by different techniques. Small angle X-ray scattering (SAXS) analysis and SEM techni...
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Redox factor-1: an extra-nuclear role in the regulation of endothelial oxidative stress and apoptosis
The rac1 GTPase promotes oxidative stress through reactive oxygen species (ROS) production, whereas the DNA repair enzyme and transcriptional regulator redox factor-1 (ref-1) protects against cell death due to...
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Book
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Chapter
Antibodies: Biochemistry, Structure, and Function
An understanding of the basic principles of the biology of the vertebrate immune system and its regulation is a prerequisite for the successful experimental production of antibodies. Such knowledge helps in av...
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Chapter
Conjugation Techniques
Preparation of well-defined conjugates during various stages, from hapten immunogen preparation for antibody production to that of tracer or labeling reagents such as enzyme-antigen or enzyme-antibody conjugat...
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Chapter
Enzymes and Signal Amplification Systems
Since the introduction of radioimmunoassays in the late 1950s and the widespread reliance on radioisotopic labels in various fields of clinical and medical science and practice during the 1960s and 1970s, rapi...
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Chapter
Immunoassay Classification and Commercial Technologies
Immunoassays encompass techniques for the detection and quantification of antigens or antibodies, and are one of the most powerful of all immunochemical techniques. Although the term “immunoassay” generally re...
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Chapter
Data Analysis
Data analysis is an important aspect of using solid-phase immunoassays. The basic principles of obtaining dose-response curves using immunoassay techniques were described earlier in this book. In quantitative ...
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Chapter
Introduction
Qualitative and quantitative analytical techniques are of tremendous importance in all fields and disciplines of life sciences for the detection, identification, and measurement of concentration of a wide vari...
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Chapter
Antigen-Antibody Reactions
Enzyme immunoassays are based on antigen-antibody reactions involving enzyme-labeled antigen (or antibody) with antibody (or antigen). An understanding of the fundamentals of antigen-antibody reactions is, the...
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Chapter
Antibody Production
The production and large scale availability of antibody of desired affinity and specificity and/or cross-reactivity are fundamental to the process of develo** sensitive, precise, and specific immunoassay pro...
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Chapter
Separation and Solid-Phase Systems
To quantitate the amount of analyte present in an enzyme immunoassay (EIA), the extent of reaction of the enzyme-labeled ligand (or antibody) with antibody (or the ligand) must be determined. Since the reactio...
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Chapter
Assay Development, Evaluation, and Validation
Modern immunoassay systems exist in several different formats with diverse ancillary technologies. An ideal immunoassay for a given need depends upon the kind of information desired and the physical environmen...
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Chapter
Reagent Formulations and Shelf Life Evaluation
The control of assay quality and the degree of quality control required begin with the source and the accurate preparation of reagents and control sera used in the assay procedure. The quality of reagents used...
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Chapter
Documentation, Registration, and Diagnostic Start-Ups
Commercial production of immunodiagnostic kits requires that the various components be manufactured consistently over a long period of time. The process involves availability of a consistent source of antibody...
