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Separation and Solid-Phase Systems

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Enzyme Immunoassays

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Abstract

To quantitate the amount of analyte present in an enzyme immunoassay (EIA), the extent of reaction of the enzyme-labeled ligand (or antibody) with antibody (or the ligand) must be determined. Since the reaction between the ligand and its specific antibody does not produce a precipitate, it is necessary to effect a physical separation of the two forms before either or both forms can be quantitated. By definition, in all heterogeneous EIAs, a physical separation of the free and antibody-bound fractions is required. The homogeneous EIAs, in contrast, are separation-free systems; they do not require separation of the free and bound forms.

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  1. Idetek, Inc., USA

    S. S. Deshpande

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© 1996 Chapman & Hall

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Deshpande, S.S. (1996). Separation and Solid-Phase Systems. In: Enzyme Immunoassays. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1169-0_7

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  • DOI: https://doi.org/10.1007/978-1-4613-1169-0_7

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  • Print ISBN: 978-1-4612-8495-6

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