Plant and Microbe Adaptations to Cold in a Changing World
Proceedings from Plant and Microbe Adaptations to Cold 2012
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In Planta Genome Editing in Commercial Wheat Varieties: Use of TaQsd1 to Lengthen Seed Dormancy
Dependency on in vitro culture and regeneration limits the ability to use genome editing on elite wheat (Triticum aestivum L.) varieties. We recently developed an in planta particle bombardment (iPB) technique fo...
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Article
Open AccessBiolistic-delivery-based transient CRISPR/Cas9 expression enables in planta genome editing in wheat
The current application of genome editing to crop plants is limited to cultivars that are amenable to in vitro culture and regeneration. Here, we report an in planta genome-editing which does not require callus c...
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Open AccessAuthor Correction: An in planta biolistic method for stable wheat transformation
A correction to this article has been published and is linked from the HTML version of this paper. The error has not been fixed in the paper.
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Article
Open AccessAn in planta biolistic method for stable wheat transformation
The currently favoured method for wheat (Triticum aestivum L.) transformation is inapplicable to many elite cultivars because it requires callus culture and regeneration. Here, we developed a simple, reproducible...
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Article
Erratum to: Interactome analysis reveals versatile functions of Arabidopsis COLD SHOCK DOMAIN PROTEIN 3 in RNA processing within the nucleus and cytoplasm
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Determination of RNA Chaperone Activity Using an Escherichia coli Mutant
Bacterial cold shock proteins (CSPs) function as RNA chaperones that destabilize RNA secondary structures. A quadruple mutant of Escherichia coli (cspA, cspB, cspG, and cspE) displays a cold-sensitive growth phen...
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Interactome analysis reveals versatile functions of Arabidopsis COLD SHOCK DOMAIN PROTEIN 3 in RNA processing within the nucleus and cytoplasm
Arabidopsis COLD SHOCK DOMAIN PROTEIN 3 (AtCSP3) shares an RNA chaperone function with E. coli cold shock proteins and regulates freezing tolerance during cold acclimation. Here, we screened for AtCSP3-interactin...
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Book and Conference Proceedings
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Article
Isolation of Anther-specific Gene Promoters Suitable for Transgene Expression in Rice
To isolate rice anther-specific promoters that allow strong and constitutive expression of transgenes under chilling stress conditions, a genome-wide screen using a microarray was conducted. Six candidate gene...
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Specific induction of TaAAPT1, an ER- and Golgi-localized ECPT-type aminoalcoholphosphotransferase, results in preferential accumulation of the phosphatidylethanolamine membrane phospholipid during cold acclimation in wheat
Cold acclimation requires substantial alteration in membrane property. In contrast to well-documented fatty acid unsaturation during cold acclimation, changes in phospholipid biosynthesis during cold acclimati...
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Subcellular Localization and Changes in mRNA Abundance of CEBP, a Nuclear-Encoded Chloroplast Protein, During Flower Development and Senescence
CEBP, a nuclear-encoded chloroplast protein, has been previously cloned as a putative transcription factor involved in ethylene signaling in carnation development and senescence. In order to more clearly defin...
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Molecular Basis of Disease Resistance Acquired through Cold Acclimation in Overwintering Plants
Plants require substantial resistance against freezing and pathogens for overwintering. These two traits are acquired through cold acclimation. In contrast to freezing tolerance, molecular basis of disease res...
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Diversification of the barley and wheat blt101/wpi6 promoters by the Xumet element without affecting stress responsiveness
BLT101-family plasma membrane proteins are found in a wide range of organisms from bacteria to nematodes and are involved in the regulation of cellular cation concentration under stress conditions. A compariso...
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A cold inducible multidomain cystatin from winter wheat inhibits growth of the snow mold fungus, Microdochium nivale
A novel cold-induced cystatin cDNA clone (TaMDC1) was isolated from cold acclimated winter wheat crown tissue by using a macroarray-based differential screening method. The deduced amino acid sequence consisted o...
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Molecular characterization of a cold-induced plasma membrane protein gene from wheat
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Molecular characterization of a cold-induced plasma membrane protein gene from wheat
As a means to study the function of plasma membrane proteins during cold acclimation, we have isolated a cDNA clone for wpi6 which encodes a putative plasma membrane protein from cold-acclimated winter wheat. The...
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Functional Identification of a Trehalose 6-phosphate Phosphatase Gene that is Involved in Transient Induction of Trehalose Biosynthesis during Chilling Stress in Rice
Trehalose serves as a stress protectant and/or reserve carbohydrate in a variety of organisms including bacteria, yeast, and invertebrates. Recently, trace amounts of trehalose have been detected in higher pla...
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Article
OsMADS22, an STMADS11-like MADS-box gene of rice, is expressed in non-vegetative tissues and its ectopic expression induces spikelet meristem indeterminacy
We report the cDNA sequence and gene expression patterns of OsMADS22, a novel member of the STMADS11-like family of MADS-box genes, from rice. In contrast to previously reported STMADS11-like genes, whose express...
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Article
Water-deficit induction of a tomato H1 histone requires abscisic acid
Many genes are induced by periods of water deficit, and a subset of these are dependent on elevated ABA content for expression. A number of drought-induced genes are not induced in leaves of the ABA-deficient ...
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Article
The coupling of differential display and AFLP approaches for nonradioactive mRNA fingerprinting
We have modified the differential display of 3′-end restriction fragments of cDNAs technique by combining it with the amplified fragment length polymorphism (AFLP) approach and silver staining. Modified oligo-...
