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Interactome analysis reveals versatile functions of Arabidopsis COLD SHOCK DOMAIN PROTEIN 3 in RNA processing within the nucleus and cytoplasm

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Cell Stress and Chaperones Aims and scope

An Erratum to this article was published on 21 January 2015

Abstract

Arabidopsis COLD SHOCK DOMAIN PROTEIN 3 (AtCSP3) shares an RNA chaperone function with E. coli cold shock proteins and regulates freezing tolerance during cold acclimation. Here, we screened for AtCSP3-interacting proteins using a yeast two-hybrid system and 38 candidate interactors were identified. Sixteen of these were further confirmed in planta interaction between AtCSP3 by a bi-molecular fluorescence complementation assay. We found that AtCSP3 interacts with CONSTANS-LIKE protein 15 and nuclear poly(A)-binding proteins in nuclear speckles. Three 60S ribosomal proteins (RPL26A, RPL40A/UBQ2, and RPL36aB) and the Gar1 RNA-binding protein interacted with AtCSP3 in the nucleolus and nucleoplasm, suggesting that AtCSP3 functions in ribosome biogenesis. Interactions with LOS2/enolase and glycine-rich RNA-binding protein 7 that are cold inducible, and an mRNA decap** protein 5 (DCP5) were observed in the cytoplasm. These data suggest that AtCSP3 participates in multiple complexes that reside in nuclear and cytoplasmic compartments and possibly regulates RNA processing and functioning.

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Abbreviations

BiFC:

Bi-molecular fluorescence complementation

COL:

CONSTANS-LIKE protein

CBF:

C-repeat-binding factor

CSD:

Cold shock domain

CSPs:

Cold shock domain protein

DCP:

Decap** protein

Gar:

Glycine-arginine rich domain

GFP:

Green fluorescent protein

GRP:

Glycine-rich RNA-binding protein

LOS:

Low expression of osmotically responsive gene

PABN:

Nuclear type of poly(A)-binding protein

RPL:

Ribosomal protein

YFP:

Yellow fluorescent protein

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Acknowledgments

This research was supported in part by grants from the Japan Society for the Promotion of Science (KAKENHI Scientific Research B 19380063) and NARO project 112g0 (Wheat and Soybean Biotechnology) to R.I.

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Authors and Affiliations

  1. Crop Breeding Research Division, Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization, Hitsujigaoka 1, Toyohira-ku, Sapporo, 062-8555, Japan

    Myung-Hee Kim, Yutaka Sonoda, Kentaro Sasaki & Ryozo Imai

  2. Laboratory of Plant Molecular Biology, Faculty of Agriculture, Tottori University, Tottori, Japan

    Hironori Kaminaka

Authors
  1. Myung-Hee Kim
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  2. Yutaka Sonoda
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  3. Kentaro Sasaki
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  4. Hironori Kaminaka
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  5. Ryozo Imai
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Correspondence to Ryozo Imai.

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An erratum to this article is available at http://dx.doi.org/10.1007/s12192-014-0567-7.

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Kim, MH., Sonoda, Y., Sasaki, K. et al. Interactome analysis reveals versatile functions of Arabidopsis COLD SHOCK DOMAIN PROTEIN 3 in RNA processing within the nucleus and cytoplasm. Cell Stress and Chaperones 18, 517–525 (2013). https://doi.org/10.1007/s12192-012-0398-3

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