37 Result(s)
within
Reuven Agami
English
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Article
Open AccessValine aminoacyl-tRNA synthetase promotes therapy resistance in melanoma
Transfer RNA dynamics contribute to cancer development through regulation of codon-specific messenger RNA translation. Specific aminoacyl-tRNA synthetases can either promote or suppress tumourigenesis. Here we...
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Article
Molecular mechanisms of non-genetic aberrant peptide production in cancer
The cancer peptidome has long been known to be altered by genetic mutations. However, more recently, non-genetic polypeptide mutations have also been related to cancer cells. These non-genetic mutations occur ...
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Article
Open AccessABPEPserver: a web application for documentation and analysis of substitutants
Cancer immunotherapy is implemented by identifying antigens that are presented on the cell surface of cancer cells and illicit T-cell response (Schumacher and Schreiber, Science 348:69–74, 2015; Waldman et al....
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Article
Open AccessSerine metabolism remodeling after platinum-based chemotherapy identifies vulnerabilities in a subgroup of resistant ovarian cancers
Resistance to platinum-based chemotherapy represents a major clinical challenge for many tumors, including epithelial ovarian cancer. Patients often experience several response-relapse events, until tumors bec...
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Article
Open AccessAuthor Correction: Tryptophan depletion results in tryptophan-to-phenylalanine substitutants
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Article
Open AccessSuppression of heparan sulfation re-sensitizes YAP1-driven melanoma to MAPK pathway inhibitors
Accumulating evidence identifies non-genetic mechanisms substantially contributing to drug resistance in cancer patients. Preclinical and clinical data implicate the transcriptional co-activators YAP1 and its ...
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Article
Open AccessTryptophan depletion results in tryptophan-to-phenylalanine substitutants
Activated T cells secrete interferon-γ, which triggers intracellular tryptophan shortage by upregulating the indoleamine 2,3-dioxygenase 1 (IDO1) enzyme1–4. Here we show that despite tryptophan depletion, in-fram...
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Article
Anti-tumour immunity induces aberrant peptide presentation in melanoma
Extensive tumour inflammation, which is reflected by high levels of infiltrating T cells and interferon-γ (IFNγ) signalling, improves the response of patients with melanoma to checkpoint immunotherapy1,2. Many tu...
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Article
Open AccessA comprehensive enhancer screen identifies TRAM2 as a key and novel mediator of YAP oncogenesis
Frequent activation of the co-transcriptional factor YAP is observed in a large number of solid tumors. Activated YAP associates with enhancer loci via TEAD4-DNA-binding protein and stimulates cancer aggressiv...
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Article
Open AccessTranslatome analysis reveals altered serine and glycine metabolism in T-cell acute lymphoblastic leukemia cells
Somatic ribosomal protein mutations have recently been described in cancer, yet their impact on cellular transcription and translation remains poorly understood. Here, we integrate mRNA sequencing, ribosome fo...
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Article
Open AccessFunctional CRISPR screen identifies AP1-associated enhancer regulating FOXF1 to modulate oncogene-induced senescence
Functional characterization of non-coding elements in the human genome is a major genomic challenge and the maturation of genome-editing technologies is revolutionizing our ability to achieve this task. Oncoge...
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Article
Long non-coding RNAs in metastasis
Long non-coding RNA (lncRNA) genes have recently been discovered as key regulators of developmental, physiological, and pathological processes in humans. Recent studies indicate that lncRNAs regulate every ste...
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Article
Open AccessFcαRI co-stimulation converts human intestinal CD103+ dendritic cells into pro-inflammatory cells through glycolytic reprogramming
CD103+ dendritic cells (DC) are crucial for regulation of intestinal tolerance in humans. However, upon infection of the lamina propria this tolerogenic response is converted to an inflammatory response. Here we ...
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Article
Characterization of noncoding regulatory DNA in the human genome
Genome-wide map** of regulatory elements will improve our understanding of how genetic variation in the noncoding genome affects disease phenotypes.
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Protocol
GRO-seq, A Tool for Identification of Transcripts Regulating Gene Expression
The advent of next-generation sequencing (NGS) technologies has revolutionized the way we do research on gene expression. High-throughput transcriptomics became possible with the development of microarray tech...
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Article
Applying CRISPR–Cas9 tools to identify and characterize transcriptional enhancers
CRISPR–Cas9-based genome editing tools have been developed recently to study non-coding transcriptional regulatory elements, enabling the characterization of enhancers in their endogenous context. The applicat...
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Article
Tumour-specific proline vulnerability uncovered by differential ribosome codon reading
Tumours can require certain amino acids for their proliferation, and the diricore method described here helps to identify such restrictive amino acids; using this method in kidney cancer tissue and breast carc...
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Article
Functional genetic screens for enhancer elements in the human genome using CRISPR-Cas9
CRISPR-Cas9 is used for both enrichment and dropout screens of functional enhancers in human cells.
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Article
Open AccessGenome-wide profiling of p53-regulated enhancer RNAs uncovers a subset of enhancers controlled by a lncRNA
p53 binds enhancers to regulate key target genes. Here, we globally mapped p53-regulated enhancers by looking at enhancer RNA (eRNA) production. Intriguingly, while many p53-induced enhancers contained p53-bin...
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Article
Open AccessRibosome profiling reveals features of normal and disease-associated mitochondrial translation
Mitochondria are essential cellular organelles for generation of energy and their dysfunction may cause diabetes, Parkinson’s disease and multi-systemic failure marked by failure to thrive, gastrointestinal pr...
