Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1543)
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About this book
This volume is divided in four sections; covering genome wide approaches, techniques for characterize of paRNA structural features are described, selecting pa-RNA, and paRNA therapeutic potential. Chapters describe how siRNAsdirected against paRNAs can be applied in vivo to modulate transcription of important genes controlled by paRNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Promoter Associated RNA: Methods and Protocols aims to demonstrate paRNAs as new class of regulatory molecules, to further investigate and value as tools for fine transcriptional tuning.
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Keywords
Table of contents (17 protocols)
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Identification of Promoter-Associated RNAs
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Characterization of Promoter-Associated RNA Features
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Functional Studies of Promoter-Associated RNAs
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Therapeutic Potential of Promoter-Associated RNAs Targeting
Editors and Affiliations
Bibliographic Information
Book Title: Promoter Associated RNA
Book Subtitle: Methods and Protocols
Editors: Sara Napoli
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6716-2
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2017
Hardcover ISBN: 978-1-4939-6714-8Published: 28 March 2017
Softcover ISBN: 978-1-4939-8281-3Published: 09 June 2018
eBook ISBN: 978-1-4939-6716-2Published: 27 March 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 287
Number of Illustrations: 101 b/w illustrations, 182 illustrations in colour
Topics: Human Genetics