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Article
Open AccessPhase separation properties of RPA combine high-affinity ssDNA binding with dynamic condensate functions at telomeres
RPA has been shown to protect single-stranded DNA (ssDNA) intermediates from instability and breakage. RPA binds ssDNA with sub-nanomolar affinity, yet dynamic turnover is required for downstream ssDNA transac...
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Article
Open AccessRPA shields inherited DNA lesions for post-mitotic DNA synthesis
The paradigm that checkpoints halt cell cycle progression for genome repair has been challenged by the recent discovery of heritable DNA lesions esca** checkpoint control. How such inherited lesions affect g...
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Article
Open AccessCHD7 and 53BP1 regulate distinct pathways for the re-ligation of DNA double-strand breaks
Chromatin structure is dynamically reorganized at multiple levels in response to DNA double-strand breaks (DSBs). Yet, how the different steps of chromatin reorganization are coordinated in space and time to d...
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Article
Open AccessEngineering Af1521 improves ADP-ribose binding and identification of ADP-ribosylated proteins
Protein ADP-ribosylation is a reversible post-translational modification that regulates important cellular functions. The identification of modified proteins has proven challenging and has mainly been achieved...
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Article
Open AccessAnalysis of PARP inhibitor toxicity by multidimensional fluorescence microscopy reveals mechanisms of sensitivity and resistance
Exploiting the full potential of anti-cancer drugs necessitates a detailed understanding of their cytotoxic effects. While standard omics approaches are limited to cell population averages, emerging single cel...
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Article
Open AccessImpact of ribonucleotide incorporation by DNA polymerases β and λ on oxidative base excision repair
Oxidative stress is a very frequent source of DNA damage. Many cellular DNA polymerases (Pols) can incorporate ribonucleotides (rNMPs) during DNA synthesis. However, whether oxidative stress-triggered DNA repa...
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Article
Macrodomain-containing proteins are new mono-ADP-ribosylhydrolases
ADP-ribosylation is an important post-translational protein modification, yet enzymes capable of removing the protein-proximal ADP-ribose were unknown. Human macrodomain proteins MacroD1, D2 and C6orf130 are n...
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Article
Transcription coactivator p300 binds PCNA and may have a role in DNA repair synthesis
The transcriptional coactivator p300 interacts with many transcription factors that participate in a broad spectrum of biological activities, such as cellular differentiation, homeostasis and growth control1,2. M...
