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Article
Open AccessRelaxed targeting rules help PIWI proteins silence transposons
In eukaryotes, small RNA guides, such as small interfering RNAs and microRNAs, direct AGO-clade Argonaute proteins to regulate gene expression and defend the genome against external threats. Only animals make ...
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Article
Defining the functions of PIWI-interacting RNAs
In the animal germ line, PIWI-interacting RNAs (piRNAs) silence transposons and may also control gene expression. Distinct, but not incompatible, models have been proposed for how piRNAs regulate genes. To pro...
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Chapter and Conference Paper
Exploring the Impact of Artificial Intelligence Learning Platforms on Interest in and Attitudes Toward Learning
Artificial Intelligence (AI) learning technologies and research in deep learning are rapidly expanding, and there is a lack of AI learning platforms to support students in related courses. At present, an under...
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Article
The evolutionarily conserved piRNA-producing locus pi6 is required for male mouse fertility
Pachytene PIWI-interacting RNAs (piRNAs), which comprise >80% of small RNAs in the adult mouse testis, have been proposed to bind and regulate target RNAs like microRNAs, cleave targets like short interfering ...
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Article
Evolutionarily conserved pachytene piRNA loci are highly divergent among modern humans
In the fetal mouse testis, PIWI-interacting RNAs (piRNAs) guide PIWI proteins to silence transposons but, after birth, most post-pubertal pachytene piRNAs map to the genome uniquely and are thought to regulate...
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Article
Cas9-mediated allelic exchange repairs compound heterozygous recessive mutations in mice
Diseases caused by different mutations in the two alleles of a gene are treated in mice by Cas-9-induced allelic exchange.
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Article
Open AccessElimination of PCR duplicates in RNA-seq and small RNA-seq using unique molecular identifiers
RNA-seq and small RNA-seq are powerful, quantitative tools to study gene regulation and function. Common high-throughput sequencing methods rely on polymerase chain reaction (PCR) to expand the starting materi...
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Article
Open AccessP53-dependent downregulation of hTERT protein expression and telomerase activity induces senescence in lung cancer cells as a result of pterostilbene treatment
Cellular senescence is characterized by permanent cell cycle arrest, triggered by a variety of stresses, such as telomerase inhibition, and it is recognized as a tumor-suppressor mechanism. In recent years, te...
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Article
Loqs and R2D2 act sequentially in the siRNA pathway in Drosophila
The Drosophila small RNA interference pathways can be triggered by dsRNA from various sources, both exogenous and endogenous. Examination of mutations in flies now shows that two RNA-binding proteins, R2D2 and Lo...
