Epidermal Growth Factor
Methods and Protocols
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Open AccessEosinophils in glioblastoma biology
Glioblastoma multiforme (GBM) is the most common primary brain tumor in adults. The development of this malignant glial lesion involves a multi-faceted process that results in a loss of genetic or epigenetic g...
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Article
Transcriptional control mechanisms associated with the nucleotide receptor P2X7, a critical regulator of immunologic, osteogenic, and neurologic functions
The nucleotide receptor P2X7 is an attractive therapeutic target and potential biomarker for multiple inflammatory and neurologic disorders, and it is expressed in several immune, osteogenic, and neurologic cell ...
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Cell signaling via the P2X7 nucleotide receptor: linkage to ROS production, gene transcription, and receptor trafficking
Extracellular nucleotides can act as important intercellular signals in diverse biological processes, including the enhanced production of factors that are key to immune response regulation. One receptor that ...
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Open AccessNucleotide receptor signalling and the generation of reactive oxygen species
Elevated levels of extracellular nucleotides are present at sites of inflammation, platelet degranulation and cellular damage or lysis. These extracellular nucleotides can lead to the activation of purinergic ...
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Protocol
Methods for Determining the Proliferation of Cells in Response to EGFR Ligands
The evaluation of cell proliferation can be accomplished by several methods. The number of cells can be determined directly by counting manually (e.g., hemocytometer) or automatically (e.g., Coulter counter or...
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Protocol
Purification and Assay of Kinase-Active EGF Receptor From Mammalian Cells by Immunoaffinity Chromatography
The epidermal growth factor (EGF) receptor possesses intrinsic protein-tyrosine kinase activity, and both overexpressed wild-type and mutated forms have been associated with many types of cancers. Therefore, u...
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Analysis of Functional Domains in the Epidermal Growth Factor Receptor Using Site-Directed Mutagenesis
Protein tyrosine kinase activity is a central feature of one class of retroviral onc genes and of several growth factor receptors including those for epidermal growth factor receptor (EGF) (Ushiro and Cohen, 1980...
