12 Result(s)
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Article
Open AccessA chromatinized origin reduces the mobility of ORC and MCM through interactions and spatial constraint
Chromatin replication involves the assembly and activity of the replisome within the nucleosomal landscape. At the core of the replisome is the Mcm2-7 complex (MCM), which is loaded onto DNA after binding to t...
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Open AccessNucleotide binding halts diffusion of the eukaryotic replicative helicase during activation
The eukaryotic replicative helicase CMG centrally orchestrates the replisome and leads the way at the front of replication forks. Understanding the motion of CMG on the DNA is therefore key to our understandin...
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Open AccessDNA replication origins retain mobile licensing proteins
DNA replication in eukaryotes initiates at many origins distributed across each chromosome. Origins are bound by the origin recognition complex (ORC), which, with Cdc6 and Cdt1, recruits and loads the Mcm2-7 (...
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Open AccessChromatin fibers stabilize nucleosomes under torsional stress
Torsional stress generated during DNA replication and transcription has been suggested to facilitate nucleosome unwrap** and thereby the progression of polymerases. However, the propagation of twist in conde...
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Open AccessCorrection: Corrigendum: Applying torque to the Escherichia coli flagellar motor using magnetic tweezers
Scientific Reports 7: Article number: 43285; published online: 07 March 2017; updated: 18 May 2018 The Acknowledgements section in this Article is incomplete. “We thank Seungkyu Ha, Yera Ye. Ussembayev, Richar...
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Open AccessApplying torque to the Escherichia coli flagellar motor using magnetic tweezers
The bacterial flagellar motor of Escherichia coli is a nanoscale rotary engine essential for bacterial propulsion. Studies on the power output of single motors rely on the measurement of motor torque and rotation...
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Correction: Corrigendum: Freely orbiting magnetic tweezers to directly monitor changes in the twist of nucleic acids
Nature Communications 2: Article number: 439 (2011); Published: 23 August 2011; Updated: 27 May 2015 The published version of this article contains two errors. In equation (18), there is a typographical error ...
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Open AccessSlow unloading leads to DNA-bound β2-sliding clamp accumulation in live Escherichia coli cells
The ubiquitous sliding clamp facilitates processivity of the replicative polymerase and acts as a platform to recruit proteins involved in replication, recombination and repair. While the dynamics of the E. coli ...
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Open AccessFreely orbiting magnetic tweezers to directly monitor changes in the twist of nucleic acids
The double-stranded nature of DNA links its replication, transcription and repair to rotational motion and torsional strain. Magnetic tweezers (MT) are a powerful single-molecule technique to apply both forces...
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Antitumour drugs impede DNA uncoiling by topoisomerase I
Topoisomerases are enzymes that act to relax supercoiling, a form of built-up strain in DNA. Topoisomerase inhibitors are important antitumour drugs, thought to act by stabilizing a covalent complex between th...
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Mesoscale conformational changes in the DNA-repair complex Rad50/Mre11/Nbs1 upon binding DNA
Atherosclerotic plaques form when blood flow is disturbed and unusual patterns of mechanical stress are applied to the blood vessel wall. The endothelial cells lining these vessels play an important role in th...
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Friction and torque govern the relaxation of DNA supercoils by eukaryotic topoisomerase IB
The manoeuvres involved in replication and transcription lead to a build-up of twisting or torsional strain in DNA, and the job of relieving the strain falls to the topoisomerases. Type IB topoisomerases (TopI...
