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Article
Open AccessA chromatinized origin reduces the mobility of ORC and MCM through interactions and spatial constraint
Chromatin replication involves the assembly and activity of the replisome within the nucleosomal landscape. At the core of the replisome is the Mcm2-7 complex (MCM), which is loaded onto DNA after binding to t...
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Article
Open AccessNucleotide binding halts diffusion of the eukaryotic replicative helicase during activation
The eukaryotic replicative helicase CMG centrally orchestrates the replisome and leads the way at the front of replication forks. Understanding the motion of CMG on the DNA is therefore key to our understandin...
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Article
Open AccessDNA replication origins retain mobile licensing proteins
DNA replication in eukaryotes initiates at many origins distributed across each chromosome. Origins are bound by the origin recognition complex (ORC), which, with Cdc6 and Cdt1, recruits and loads the Mcm2-7 (...
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Protocol
Imaging of DNA and Protein by SFM and Combined SFM-TIRF Microscopy
Direct imaging is invaluable for understanding the mechanism of complex genome transactions where proteins work together to organize, transcribe, replicate and repair DNA. Scanning (or atomic) force microscopy...
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Article
Open AccessTumor slice culture system to assess drug response of primary breast cancer
The high incidence of breast cancer has sparked the development of novel targeted and personalized therapies. Personalization of cancer treatment requires reliable prediction of chemotherapy responses in indiv...
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Article
Open AccessSFMetrics: an analysis tool for scanning force microscopy images of biomolecules
Scanning force microscopy (SFM) allows direct, rapid and high-resolution visualization of single molecular complexes; irregular shapes and differences in sizes are immediately revealed by the scanning tip in t...
