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  1. Article

    Open Access

    Characterizing microfluidic approaches for a fast and efficient reagent exchange in single-molecule studies

    Single-molecule experiments usually take place in flow cells. This experimental approach is essential for experiments requiring a liquid environment, but is also useful to allow the exchange of reagents before...

    Julene Madariaga-Marcos, Roberta Corti, Silvia Hormeño in Scientific Reports (2020)

  2. No Access

    Protocol

    High-Resolution Atomic Force Microscopy Imaging of Nucleic Acids

    Exploring the limits of spatial resolution has been a constant in the history of atomic force microscopy imaging. Since its invention in 1986, the AFM has beaten the barrier of resolution continuously, thanks ...

    Pablo Ares, Julio Gomez-Herrero, Fernando Moreno-Herrero in Nanoscale Imaging (2018)

  3. Article

    Open Access

    TubZ filament assembly dynamics requires the flexible C-terminal tail

    Cytomotive filaments are essential for the spatial organization in cells, showing a dynamic behavior based on nucleotide hydrolysis. TubZ is a tubulin-like protein that functions in extrachromosomal DNA moveme...

    Maria E. Fuentes-Pérez, Rafael Núñez-Ramírez in Scientific Reports (2017)

  4. No Access

    Article

    High flexibility of DNA on short length scales probed by atomic force microscopy

    The mechanics of DNA bending on intermediate length scales (5–100 nm) plays a key role in many cellular processes, and is also important in the fabrication of artificial DNA structures, but previous experiment...

    Paul A. Wiggins, Thijn van der Heijden, Fernando Moreno-Herrero in Nature Nanotechnology (2006)

  5. No Access

    Article

    Mesoscale conformational changes in the DNA-repair complex Rad50/Mre11/Nbs1 upon binding DNA

    Atherosclerotic plaques form when blood flow is disturbed and unusual patterns of mechanical stress are applied to the blood vessel wall. The endothelial cells lining these vessels play an important role in th...

    Fernando Moreno-Herrero, Martijn de Jager, Nynke H. Dekker, Roland Kanaar in Nature (2005)