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Article
Open AccessCellular heterogeneity of pluripotent stem cell-derived cardiomyocyte grafts is mechanistically linked to treatable arrhythmias
Preclinical data have confirmed that human pluripotent stem cell-derived cardiomyocytes (PSC-CMs) can remuscularize the injured or diseased heart, with several clinical trials now in planning or recruitment st...
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Article
Open AccessA village in a dish model system for population-scale hiPSC studies
The mechanisms by which DNA alleles contribute to disease risk, drug response, and other human phenotypes are highly context-specific, varying across cell types and different conditions. Human induced pluripot...
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Chapter
Analysing Genetic Programs of Cell Differentiation to Study Cardiac Cell Diversification
Developmental cell lineages are first specified at the point of gastrulation, a process involving precise control of timing and dosage of signalling pathways required to guide differentiation into all cell typ...
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Article
Open AccessElp2 mutations perturb the epitranscriptome and lead to a complex neurodevelopmental phenotype
Intellectual disability (ID) and autism spectrum disorder (ASD) are the most common neurodevelopmental disorders and are characterized by substantial impairment in intellectual and adaptive functioning, with t...
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Article
Open AccessSingle cell eQTL analysis identifies cell type-specific genetic control of gene expression in fibroblasts and reprogrammed induced pluripotent stem cells
The discovery that somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) has provided a foundation for in vitro human disease modelling, drug development and population genetics studies. ...
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Article
Open AccessGenotype-free demultiplexing of pooled single-cell RNA-seq
A variety of methods have been developed to demultiplex pooled samples in a single cell RNA sequencing (scRNA-seq) experiment which either require hashtag barcodes or sample genotypes prior to pooling. We intr...
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Article
Open AccessA transcribed enhancer dictates mesendoderm specification in pluripotency
Enhancers and long noncoding RNAs (lncRNAs) are key determinants of lineage specification during development. Here, we evaluate remodeling of the enhancer landscape and modulation of the lncRNA transcriptome d...
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Article
Generating high-purity cardiac and endothelial derivatives from patterned mesoderm using human pluripotent stem cells
Different concentrations of activin A and BMP4 are used to polarize stem cells into mesodermal subtypes that reflect mid-primitive-streak cardiogenic mesoderm and posterior-primitive-streak hemogenic mesoderm.
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Article
Open AccessErratum to: Statistically based splicing detection reveals neural enrichment and tissue-specific induction of circular RNA during human fetal development
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Article
Open AccessStatistically based splicing detection reveals neural enrichment and tissue-specific induction of circular RNA during human fetal development
The pervasive expression of circular RNA is a recently discovered feature of gene expression in highly diverged eukaryotes, but the functions of most circular RNAs are still unknown. Computational methods to d...
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Article
Human embryonic-stem-cell-derived cardiomyocytes regenerate non-human primate hearts
Regeneration of the heart muscle after myocardial infarction with cardiomyocytes derived from human embryonic stem cells is demonstrated in non-human primates, with the grafts showing evidence of electromechan...
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Protocol
Methods for Assessing the Electromechanical Integration of Human Pluripotent Stem Cell-Derived Cardiomyocyte Grafts
Cardiomyocytes derived from human pluripotent stem cells show tremendous promise for the replacement of myocardium and contractile function lost to infarction. However, until recently, no methods were availabl...
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Article
Human ES-cell-derived cardiomyocytes electrically couple and suppress arrhythmias in injured hearts
A guinea-pig model of cardiac injury is used to show that human embryonic stem-cell-derived cardiomyocyte grafts can electrically integrate into the injured heart, improving mechanical function and reducing sp...
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Article
Reprogramming the injured heart
When the heart is injured, the muscle does not regenerate and scars are produced. This process can be attenuated in the hearts of live mice by forcing scar-forming cells to become muscle cells. See Articles ...
