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Article
Involvement of the Mitochondrial Ca2+-Independent Phospholipase iPLA2 in the Induction of Mitochondrial Permeability Transition Pore by Long-Chain Acylcarnitines
It is known that activated derivatives of long-chain fatty acids acylcarnitines (LCAC) are considered the most toxic, which, along with calcium, can participate in the induction of the mitochondrial pore, invo...
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Article
Induction of Mitochondrial Cyclosporin-Dependent Permeability Transition Pore by Acylcarnitines. Effects of Concentrations and Carbon Chain Length
It is known that the concentrations of long-chain fatty acids (LCFA) and their carnitine derivatives (LCAC) in obesity and diabetes greatly increase, and under conditions of ischemia-reperfusion can exceed ten...
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Article
The Effects of L-Arginine and Nitric Oxide Donors on the Induction of Mitochondrial Permeability Transition Pore by Calcium Ions and Palmitoylcarnitine. Possible Involvement of Mitochondrial NO/cGMP/PKG Signaling System
This study was focused on the mechanisms of the protective effects of L-arginine and the nitric oxide donor SNAP under the induction of cyclosporine A-dependent mitochondrial permeability transition pore (MPTP) b...
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Article
Synthesis and Cytostatic Activity of New Mepregenol 17-Acetate Derivatives with Respect to Hela Cancer Cell Culture
New mepregenol 17-acetate derivatives with potential cytotoxicity were synthesized. Mepregenol 17-acetate 3-acrylate (III) was shown to exhibit potent cytotoxicity although the ester of N-benzylpyrrolidine-3-carb...
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Article
Implication of Mitochondrial NO/cGMP/PKG Signaling System in the Activation and Inhibition of Mitochondrial Respiration by L-Arginine and NO Donors
The involvement of the mitochondrial calcium-dependent NO synthase (mtNOS) in the regulation of mitochondrial respiration has not been sufficiently studied. Moreover, the possible functioning of a mitochondria...
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Article
Preparation of a Horse Chestnut Extract with a 50% Content of Escin and its Actions on Tumor Cell Proliferation and Isolated Mitochondria
Escin-containing horse chestnut extracts and various medicinal formulations are widely used as venotonic agents whose mechanisms of action and intracellular targets remain unknown. Escin is used as an active s...
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Article
The Effect of Microbial Metabolites on the Functions of Mitochondria in Acidosis and Deficiency of the Substrates of Oxidation
Mitochondrial dysfunction is the main factor in the development of multiple organ failure in sepsis. The role of microbial metabolites in the induction of mitochondrial dysfunction was studied in this work. Th...
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Article
Modulation of the Activity of Succinate Dehydrogenase by Acetylation with Chemicals, Drugs, and Microbial Metabolites
Abstract—The effects of acetylating and deacetylating compounds on the activity of succinate dehydrogenase, as well as on the membrane potential and calcium retention capacity of the isolated liver mitochondria, ...
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Article
Preparation of Nuflein - an Alkaloid from the Yellow Waterlily Nuphar Lutea - and its Cytotoxic Action on Cultures of Normal and Tumorous Human Cells
The alkaloid from the yellow waterlily nuflein, whose preparation is described here, has cytotoxic activity against HeLa cervical cancer tumor cells. Suppression of the viability of normal fibroblasts requires...
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Article
Substrate-specific reduction of tetrazolium salts by isolated mitochondria, tissues, and leukocytes
Tetrazolium salts are commonly used in cytochemical and biochemical studies as indicators of metabolic activity of cells. Formazans, formed by reduction of tetrazolium salts, behave as pseudo-solutions during ...
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Article
The effect of cell-phone radiation on rabbits: Lymphocyte enzyme-activity data
The effect of a GSM 900/1800 mobile phone, which is a widespread source of electromagnetic radiation of the microwave frequency in the environment, on rabbits was studied at power densities of 5–7 μW/cm2. The bio...
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Article
Induction of cyclosporine-sensitive mitochondrial permeability transition pore by substrates forming acetyl-CoA under normal conditions and in type 2 diabetes
Oxidation of pyruvate and palmitoylcarnitine in mitochondria is accompanied by the formation of acetyl-CoA, with its possible participation in the acetylation of various proteins and enzymes that may lead to t...
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Article
The effects of near infrared radiation on rats assessed by succinate dehydrogenase activity in lymphocytes in blood smears
The biological effects of near infrared radiation (850 nm) modulated by an acoustic frequency of 101 Hz were studied. The study was conducted on rats; the effect was registered by succinate dehydrogenase activ...
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Article
The effects of phenolic microbial metabolites on the activities of mitochondrial enzymes
The effects of microbial phenolic metabolites on the activities of enzymes of the tricarboxylic acid cycle were investigated in isolated mitochondria. The detection of metabolites of the tricarboxylic acid cyc...
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Article
Changes in lymphocytes induced by oxidation substrates
Lymphocytes in blood smears of children diagnosed with cancer have been investigated and compared to those of healthy children (matching controls). The conventional hematological procedure for smear staining w...
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Article
Redox-dependent ferric oxide nanoparticles loaded with doxorubicin and their influence on the functions of mitochondria
The spectral, fluorescent and functional properties of ferric oxide and ferric hydroxide nanoparticles loaded with doxorubicin and stabilized with citric acid or lysine were studied in comparison with free dox...
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Article
Mitochondrial models of pathologies with oxidative stress. Efficiency of alkalization to reduce mitochondrial damage
Previously, we developed a method to monitor the development of oxidative stress in isolated liver mitochondria. The method is based on recording of membrane potential changes in response to sequential introdu...
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Article
Mechanism of induction of oxidative stress in liver mitochondria by low concentrations of tert-butyl hydroperoxide
The mechanism of the effect of tert-butyl hydroperoxide (tBHP) on the kinetics of decrease in liver mitochondrial ΔΨ (transmembrane electric potential) in response to successive additions of tBHP in low concentra...
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Article
States of succinate dehydrogenase in the organism: Dormant vs. hyperactive (pushed out of equilibrium)
Using an original cytobiochemical method to study oxidation in mitochondria, preserving their native network organization within cells in a blood smear, we have revealed a hyperactive state of succinate dehydr...
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Article
Effect of steroid hormones on production of reactive oxygen species in mitochondria
Among the targets of steroid hormones are mitochondria, which as the main source of reactive oxygen species (ROS) in the cell play a central role in the development of various pathologies. We studied the effec...
