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Article
Open AccessIdentification of endothelial and mesenchymal FOXF1 enhancers involved in alveolar capillary dysplasia
Mutations in the FOXF1 gene, a key transcriptional regulator of pulmonary vascular development, cause Alveolar Capillary Dysplasia with Misalignment of Pulmonary Veins, a lethal lung disease affecting newborns an...
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Article
Open AccessGuided construction of single cell reference for human and mouse lung
Accurate cell type identification is a key and rate-limiting step in single-cell data analysis. Single-cell references with comprehensive cell types, reproducible and functionally validated cell identities, an...
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Article
Open AccessAn integrated cell atlas of the lung in health and disease
Single-cell technologies have transformed our understanding of human tissues. Yet, studies typically capture only a limited number of donors and disagree on cell type definitions. Integrating many single-cell ...
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Article
Open AccessCRISPRi-mediated functional analysis of NKX2-1-binding sites in the lung
The transcription factor NKX2-1/TTF-1 is involved in lung pathophysiology, including breathing, innate defense and tumorigenesis. To understand the mechanism by which NKX2-1 regulates genes involved in such pa...
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Article
Open AccessClinical application of a lung cancer organoid (tumoroid) culture system
Despite high expectations for lung tumoroids, they have not been applied in the clinic due to the difficulty of their long-term culture. Here, however, using AO (airway organoid) media developed by the Clevers...
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Article
Single-cell meta-analysis of SARS-CoV-2 entry genes across tissues and demographics
Angiotensin-converting enzyme 2 (ACE2) and accessory proteases (TMPRSS2 and CTSL) are needed for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) cellular entry, and their expression may shed light...
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Article
Open AccessSingle cell RNA analysis identifies cellular heterogeneity and adaptive responses of the lung at birth
The respiratory system undergoes a diversity of structural, biochemical, and functional changes necessary for adaptation to air breathing at birth. To identify the heterogeneity of pulmonary cell types and dyn...
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Article
An EGFR ligand promotes EGFR-mutant but not KRAS-mutant lung cancer in vivo
EGFR ligands (e.g., EGF and TGFA) have been shown to be clinically associated with poor survival in lung cancer. Since TGFA itself initiates autochthonous tumors in liver, breast, and pancreas but not in the l...
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Protocol
Single-Cell Transcriptome Analysis Using SINCERA Pipeline
Genome-scale single-cell biology has recently emerged as a powerful technology with important implications for both basic and medical research. There are urgent needs for the development of computational metho...
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Article
Open AccessSOX2 suppresses CDKN1A to sustain growth of lung squamous cell carcinoma
Since the SOX2 amplification was identified in lung squamous cell carcinoma (lung SCC), SOX2 transcriptional downstream targets have been actively investigated; however, such targets are often cell line specific....
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Article
Measuring and ranking attacks based on vulnerability analysis
As the number of software vulnerabilities increases, the research on software vulnerabilities becomes a focusing point in information security. A vulnerability could be exploited to attack the information asse...
