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Article
Outcomes of the EMDataResource cryo-EM Ligand Modeling Challenge
The EMDataResource Ligand Model Challenge aimed to assess the reliability and reproducibility of modeling ligands bound to protein and protein–nucleic acid complexes in cryogenic electron microscopy (cryo-EM) ...
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Article
Improving resolution and resolvability of single-particle cryoEM structures using Gaussian mixture models
Cryogenic electron microscopy is widely used in structural biology, but its resolution is often limited by the dynamics of the macromolecule. Here we developed a refinement protocol based on Gaussian mixture m...
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Article
Chikungunya virus assembly and budding visualized in situ using cryogenic electron tomography
Chikungunya virus (CHIKV) is a representative alphavirus causing debilitating arthritogenic disease in humans. Alphavirus particles assemble into two icosahedral layers: the glycoprotein spike shell embedded i...
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Article
Open AccessCryo-EM model validation recommendations based on outcomes of the 2019 EMDataResource challenge
This paper describes outcomes of the 2019 Cryo-EM Model Challenge. The goals were to (1) assess the quality of models that can be produced from cryogenic electron microscopy (cryo-EM) maps using current modeli...
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Article
Open AccessResolving individual atoms of protein complex by cryo-electron microscopy
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Article
Measurement of atom resolvability in cryo-EM maps with Q-scores
Cryogenic electron microscopy (cryo-EM) maps are now at the point where resolvability of individual atoms can be achieved. However, resolvability is not necessarily uniform throughout the map. We introduce a q...
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Article
Open AccessCoupling of ssRNA cleavage with DNase activity in type III-A CRISPR-Csm revealed by cryo-EM and biochemistry
The type III CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR-associated genes) systems are bacterially encoded adaptive immune systems for defense against invading nucleic acids. T...
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Article
Convolutional neural networks for automated annotation of cellular cryo-electron tomograms
An algorithm and software tool automating the annotation of subcellular features in cryo-electron tomography data is presented.
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Article
Zernike phase-contrast electron cryotomography applied to marine cyanobacteria infected with cyanophages
Equip** an electron microscope with Zernike phase-contrast optics dramatically increases the contrast of the images. Dai et al. describe how to successfully apply this technology for the acquisition and analysi...
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Article
Structural changes in a marine podovirus associated with release of its genome into Prochlorococcus
Single particle cryo-electron microscopy reconstructions of marine podovirus particles, together with cryo-electron tomography studies during the host infection process, have revealed structural features that ...
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Article
Pushing back the limits of electron cryomicroscopy
A convergence of technical advances in electron cryomicroscopy will allow visualization of biomolecular assemblies, without crystals, at resolutions below 10 Å. Recent cryomicroscopic structures of papillomavi...