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Article
Multilayered VBC score predicts sgRNAs that efficiently generate loss-of-function alleles
CRISPR–Cas9 screens have emerged as a transformative approach to systematically probe gene functions. The quality and success of these screens depends on the frequencies of loss-of-function alleles, particular...
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Article
Open AccessCRISPR-Switch regulates sgRNA activity by Cre recombination for sequential editing of two loci
CRISPR-Cas9 is an efficient and versatile tool for genome engineering in many species. However, inducible CRISPR-Cas9 editing systems that regulate Cas9 activity or sgRNA expression often suffer from significa...
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Article
CRISPR-UMI: single-cell lineage tracing of pooled CRISPR–Cas9 screens
Combining each sgRNA with a unique molecular identifier in a genome-wide screen increases sensitivity and robustness in both positive and negative selection.
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Article
A reversible haploid mouse embryonic stem cell biobank resource for functional genomics
The Haplobank contains over 100,000 individually reversibly mutagenized, barcoded, mouse embryonic cell lines; proof-of-principle experiments were used to search for genes that are required for rhinovirus infe...
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Article
The histone chaperone CAF-1 safeguards somatic cell identity
Cellular differentiation involves profound remodelling of chromatic landscapes, yet the mechanisms by which somatic cell identity is subsequently maintained remain incompletely understood. To further elucidate...
