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  1. Article

    Open Access

    A Type II-B Cas9 nuclease with minimized off-targets and reduced chromosomal translocations in vivo

    Streptococcus pyogenes Cas9 (SpCas9) and derived enzymes are widely used as genome editors, but their promiscuous nuclease activity often induces undesired mutations and chromosomal rearrangements. Several strate...

    Burcu Bestas, Sandra Wimberger, Dmitrii Degtev, Alexandra Madsen… in Nature Communications (2023)

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  2. No Access

    Article

    Bridge helix arginines play a critical role in Cas9 sensitivity to mismatches

    The RNA-programmable DNA-endonuclease Cas9 is widely used for genome engineering, where a high degree of specificity is required. To investigate which features of Cas9 determine the sensitivity to mismatches a...

    Majda Bratovič, Ines Fonfara, Krzysztof Chylinski… in Nature Chemical Biology (2020)

  3. Article

    Open Access

    CRISPR-Switch regulates sgRNA activity by Cre recombination for sequential editing of two loci

    CRISPR-Cas9 is an efficient and versatile tool for genome engineering in many species. However, inducible CRISPR-Cas9 editing systems that regulate Cas9 activity or sgRNA expression often suffer from significa...

    Krzysztof Chylinski, Maria Hubmann, Ruth E. Hanna, Connor Yanchus… in Nature Communications (2019)

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  4. No Access

    Article

    CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

    CRISPR/Cas systems constitute a widespread class of immunity systems that protect bacteria and archaea against phages and plasmids, and commonly use repeat/spacer-derived short crRNAs to silence foreign nuclei...

    Elitza Deltcheva, Krzysztof Chylinski, Cynthia M. Sharma, Karine Gonzales… in Nature (2011)