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Article
Biocatalytic desymmetrization for synthesis of chiral enones using flavoenzymes
Efficient methods for achieving desaturation of carbonyl compounds are highly sought after in organic chemistry. In contrast to synthetic approaches, enzymatic desaturation systems offer the potential to enhan...
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Article
Publisher Correction: Engineering non-haem iron enzymes for enantioselective C(sp3)–F bond formation via radical fluorine transfer
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Article
Biocatalytic strategy for the construction of sp3-rich polycyclic compounds from directed evolution and computational modelling
Catalysis with engineered enzymes has provided more efficient routes for the production of active pharmaceutical agents. However, the potential of biocatalysis to assist in early-stage drug discovery campaigns...
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Article
Engineering non-haem iron enzymes for enantioselective C(sp3)–F bond formation via radical fluorine transfer
In recent years there has been a surge in the development of methods for the synthesis of organofluorine compounds. However, enzymatic methods for C–F bond formation have been limited to nucleophilic fluoride ...
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Article
Photobiocatalysts tame nitrogen-centred radicals
Enzymes are capable of controlling the reactivity and selectivity of catalytic mechanisms involving highly reactive intermediates. Now, flavine mononucleotide-dependent ene-reductases have been repurposed as p...
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Article
Open AccessEngineered cytochrome P450 for direct arylalkene-to-ketone oxidation via highly reactive carbocation intermediates
Ketones are crucial intermediates in synthesis and frequent moieties in many products. The direct regioselective synthesis of ketones from internal alkenes could simplify synthetic routes and solve a long-stan...
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Article
Mechanistic and structural characterization of an iridium-containing cytochrome reveals kinetically relevant cofactor dynamics
Artificial metalloenzymes (ArMs), which contain non-native, typically synthetic, metal cofactors, are a flourishing class of biocatalyst for unnatural reactions. Although the number of these reactions is rapid...
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Article
Dual-function enzyme catalysis for enantioselective carbon–nitrogen bond formation
Chiral amines can be made by insertion of a carbene into an N–H bond using two-catalyst systems that combine a transition metal-based carbene-transfer catalyst and a chiral proton-transfer catalyst to enforce ...
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Article
Open AccessPervasive cooperative mutational effects on multiple catalytic enzyme traits emerge via long-range conformational dynamics
Multidimensional fitness landscapes provide insights into the molecular basis of laboratory and natural evolution. To date, such efforts usually focus on limited protein families and a single enzyme trait, wit...
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Article
Open AccessA promiscuous cytochrome P450 aromatic O-demethylase for lignin bioconversion
Microbial aromatic catabolism offers a promising approach to convert lignin, a vast source of renewable carbon, into useful products. Aryl-O-demethylation is an essential biochemical reaction to ultimately catabo...
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Article
Structural basis of the Cope rearrangement and cyclization in hapalindole biogenesis
Hapalindole alkaloids are a structurally diverse class of cyanobacterial natural products defined by their varied polycyclic ring systems and diverse biological activities. These complex metabolites are genera...
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Article
Enzyme-catalyzed cationic epoxide rearrangements in quinolone alkaloid biosynthesis
Discovery and characterization of two Brønsted acid enzymes from quinolone alkaloid biosynthesis provides new biocatalytic approaches for the challenging but synthetically useful cationic rearrangement of epox...
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Chapter
Understanding the Exohedral Functionalization of Endohedral Metallofullerenes
The endohedral metallofullerenes (EMFs) and their exohedral functionalized derivatives present an increasing attention due to their potential applications in materials science and medicine. However, the curren...
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Article
Sponge-like molecular cage for purification of fullerenes
Since fullerenes are available in macroscopic quantities from fullerene soot, large efforts have been geared toward designing efficient strategies to obtain highly pure fullerenes, which can be subsequently ap...
