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Article
Open AccessSerum sodium improvement: change in Comprehensive Geriatric Assessment parameters in geriatric patients with hyponatremia
Hyponatremia presents with symptoms considered age-associated in the elderly. We assess the change in Comprehensive Geriatric Assessment (CGA) parameters after hyponatremia improvement in hospitalized geriatri...
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Article
Genome-wide association study identifies a novel locus for cannabis dependence
Despite moderate heritability, only one study has identified genome-wide significant loci for cannabis-related phenotypes. We conducted meta-analyses of genome-wide association study data on 2080 cannabis-depe...
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Article
Ventral striatal regulation of CREM mediates impulsive action and drug addiction vulnerability
Impulsivity, a multifaceted behavioral hallmark of attention-deficit/hyperactivity disorder (ADHD), strongly influences addiction vulnerability and other psychiatric disorders that incur enormous medical and s...
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Article
An endophenotype approach to the genetics of alcohol dependence: a genome wide association study of fast beta EEG in families of African ancestry
Fast beta (20–28 Hz) electroencephalogram (EEG) oscillatory activity may be a useful endophenotype for studying the genetics of disorders characterized by neural hyperexcitability, including substance use diso...
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Article
Evidence of CNIH3 involvement in opioid dependence
Opioid dependence, a severe addictive disorder and major societal problem, has been demonstrated to be moderately heritable. We conducted a genome-wide association study in Comorbidity and Trauma Study data co...
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Article
Open AccessGenome-wide polygenic scores for age at onset of alcohol dependence and association with alcohol-related measures
Age at onset of alcohol dependence (AO-AD) is a defining feature of multiple drinking typologies. AO-AD is heritable and likely shares genetic liability with other aspects of alcohol consumption. We examine wh...
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Article
A genome-wide association study of alcohol-dependence symptom counts in extended pedigrees identifies C15orf53
Several studies have identified genes associated with alcohol-use disorders (AUDs), but the variation in each of these genes explains only a small portion of the genetic vulnerability. The goal of the present ...
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Article
Statistical optimization of alkaline xylanase production from Streptomyces violaceoruber under submerged fermentation using response surface methodology
Response surface methodology employing central composite design (CCD) was used to optimize fermentation medium for the production of cellulase-free, alkaline xylanase from Streptomyces violaceoruber under submerg...
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Article
Global detection of molecular changes reveals concurrent alteration of several biological pathways in nonsmall cell lung cancer cells
To identify the molecular changes that occur in non-small cell lung carcinoma (NSCLC), we compared the gene expression profile of the NCI-H292 (H292) NSCLC cell line with that of normal human tracheobronchial ...
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Article
The role of antioxidants in models of inflammation: Emphasis on l-arginine and arachidonic acid metabolism
Inflammatory processes are made up of a multitude of complex cascades. Under physiological conditions these processes aid in tissue repair. However, under pathophysiological environments, such as wound healing...
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Article
High constitutive peroxidase activity and constitutive thermotolerance in Neurospora crassa
During the isolation of mutations in the heat-inducible hsp70-1 gene of Neurospora crassa by RIP (repeat-induced point mutations), several transformants were generated by electroporation of conidia with a plasmi...
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Article
p53 and Fas ligand are required for psoralen and UVA-induced apoptosis in mouse epidermal cells
A combination of 8-methoxypsoralen (8-MOP) and ultraviolet-A (UVA) radiation (320–400 nm) (PUVA) is widely used in the treatment of psoriasis and other skin diseases. PUVA is highly effective in eliminating hy...
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Article
Induction profiles and properties of a novel stress-induced peroxidase in Neurospora crassa
Exposure of Neurospora crassa cells to heat shock and oxidative stress results in the synthesis of several stress-related proteins, including a peroxidase. Northern blot analysis of total RNA revealed a heat-ind...
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Article
Microbial xylanases and their industrial applications: a review
Despite an increased knowledge of microbial xylanolytic systems in the past few years, further studies are required to achieve a complete understanding of the mechanism of xylan degradation by microorganisms ...
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Article
Production and characterization of thermostable xylanase and pectinase from Streptomyces sp. QG-11-3
Streptomyces sp. QG-11-3, which produces a cellulase-free thermostable xylanase (96 IU ml−1) and a pectinase (46 IU ml−1), was isolated on Horikoshi medium supplemented with 1% w/v wheat bran. Carbon sources that...
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Article
Organization of chloroplast ribosomal RNA genes and in vitro self-splicing activity of the large subunit rRNA intron from the green alga Chlorella vulgaris C-27
Sequencing of the rRNA gene (rrn) cluster of Chlorella vulgaris C-27 chloroplasts has revealed a striking organizational difference in comparison to another species of the same genus, Chlorella ellipsoidea C-87....
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Article
Sequence repeat-induced disruption of the major heat-inducible HSP70 gene of Neurospora crassa
The process of repeat-induced point mutation (RIP) was used to disrupt hsps-1, the gene encoding the major heat-inducible member of the HSP70 family of Neurospora crassa. A plasmid DNA, containing an incomplete c...
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Protocol
Gene Transfer by Electroporation of Filamentous Fungi
The fungi encompass an enormous array of species, ranging from microscopic uninucleate, unicellular forms to multinucleate, coenocytic, highly differentiated macroscopic morphological forms. The wealth of dive...
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Article
Changes in the levels of peroxidase and phenylalanine ammonia-lyase inBrassica napus cultivars showing variable resistance toLeptosphaeria maculans
Peroxidase and phenylalanine ammonia-lyase activities (PAL) were determined in leaves of healthy and inoculatedBrassica napus cultivars, showing differential disease reaction towards a virulent and a weakly virul...
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Article
A Neurospora crassa heat-shocked cell lysate translates homologous and heterologous messenger RNA efficiently, without preference for heat shock messages
Cell-free protein synthesis systems were prepared from normally-grown (N-lysate) and heat-shocked (HS-lysate) Neurospora crassa mycelium. Although both lysates translated homologous mRNA, the HS-lysate was more a...
