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Article
A novel, ultra-large xylanolytic complex (xylanosome) secreted by Streptomyces olivaceoviridis
The production of a novel, very large xylanolytic complex (xylanosome) by Streptomyces olivaceoviridis E-86 is reported. The molecular weight was approx. 1200 kDa as determined by native gradient gel electrophore...
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Article
Fast screening target sites for RNA interference using a cell-free system
A fast and simple procedure to screen target sites for RNA interference by using RNA in a cell-free system of Hela cells, and then evaluating the efficiency by Northern blotting, is described. This procedure p...
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Article
Lichenicolins A and B, New Bisnaphthopyrones from an Unidentified Lichenicolous Fungus, Strain LL-RB0668
Lichenicolous fungus LL-RB0668 was isolated from a processed lichen thallus on a modified Lilly-Barnett solid medium. Two new bisnaphthopyrone compounds, lichenicolins A (1) and B (2), were isolated from the cult...
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Article
Mathematical Model and Mechanisms for Biofilm Wastewater Treatment Systems
Fundamental theoretical depiction is still lacking on biofilm wastewater treatment systems up to today. A mathematical model of biofilm wastewater treatment systems, taking account of suspended microorganisms ...
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Article
Protein kinase Cζ and glucose uptake
Protein kinase Cζ (PKCζ) is a member of the PKC family, serving downstream of insulin receptor and phosphatidylinositol (PI) 3-kinase. Many evidences suggest that PKCζ plays a very important role in activating...
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Article
Production of fungal antibiotics using polymeric solid supports in solid-state and liquid fermentation
The use of inert absorbent polymeric supports for cellular attachment in solid-state fungal fermentation influenced growth, morphology, and production of bioactive secondary metabolites. Two filamentous fungi ...
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Article
Microbial Ecological Model of Filamentous Bulking and Mechanisms
The paper proposes a prototype mathematical model for the interactions among filamentous bacteria, floc-forming bacteria and protozoa within completely mixed activated sludge systems, which is capable of descr...
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Article
Effects of ammonia on hydrolysis of proteins and lipids from fish residues
This study investigated the influence of ammonia on the hydrolysis rates of proteins and lipids in fish residues under mesophilic anaerobic incubation at a neutral pH. The hydrolysis kinetics of the fish resid...
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Article
Isolation and characterization of a novel cDNA encoding ERF/AP2-type transcription factor OsAP25 from Oryza Sativa L.
Using a yeast one-hybrid method, a transcription factor, OsAP25, which interacts specifically with a GCC box was isolated from rice. The OsAP25 protein contained a conserved ethylene-responsive element binding...
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Article
Enzyme activities in activated sludge flocs
This study quantified the activities of enzymes in extracellular polymeric substances (EPS) and in pellets. Seven commonly adopted extraction schemes were utilized to extract from aerobic flocs the contained E...
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Article
Lipase-catalyzed transesterification of soybean oil for biodiesel production in tert-amyl alcohol
Lipase-catalyzed transesterification of soybean oil and methanol for biodiesel production in tert-amyl alcohol was investigated. The effects of different organic medium, molar ratio of substrate, reaction tempera...
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Article
Strain improvement of Serratia marcescens ECU1010 and medium cost reduction for economic production of lipase
Industrial strain improvement plays a central role in the commercial development of microbial fermentation processes. The strain of Serratia marcescens ECU1010, a wild-type lipase-producer capable of stereospecif...
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Article
Antimycin A18 produced by an endophytic Streptomyces albidoflavus isolated from a mangrove plant
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Article
Rubellimicrobium roseum sp. nov., a Gram-negative bacterium isolated from the forest soil sample
A novel pink-coloured, non-spore-forming, non-motile, Gram-negative bacterium, designated YIM 48858T, is described by using a polyphasic approach. The strain can grow at pH 6.5–9 (optimum at pH 7) and 25–30°C (op...
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Article
Methylobacterium soli sp. nov. a methanol-utilizing bacterium isolated from the forest soil
A Gram-negative, pink-pigmented, non-spore-forming rod shaped, methanol-utilizing bacterium, strain YIM 48816T, was isolated from forest soil collected from Sichuan province, China. Strain YIM 48816T can grow at ...
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Article
Rapid detection of ethambutol-resistant genes in Mycobacterium tuberculosis clinical isolates
An oligonucleotide probe has been designed and compounded for detecting the ethambutol (EMB) resistance gene of Mycobacterium tuberculosis. It was placed on a nitrocellulose membrane for use in the reverse dot-bl...
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Article
Catalytic characteristics of plant-esterase from wheat flour
A plant-esterase extracted from wheat flour and purified with a PEG1000/NaH2PO4 aqueous two-phase system was characterized for its catalytic characteristics. The optimal condition for plant-esterase to catalyze 1...
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Article
Optimization of fermentation process for the production of intracellular polysaccharide from Paecilomyces cicadae and the immuno-stimulating activity of intracellular polysaccharide
Optimization of fermentation process for the production of intracellular polysaccharide (IPS) from the fungus Paecilomyces cicadae and the immuno-stimulating activity of IPS were carried out. The quantitative eff...
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Article
Heterologous overexpression of Vigna radiata epoxide hydrolase in Escherichia coli and its catalytic performance in enantioconvergent hydrolysis of p-nitrostyrene oxide into (R)-p-nitrophenyl glycol
Two native epoxide hydrolases (EHs) were previously discovered from mung bean powder (Vigna radiata), both of which can catalyze the enantioconvergent hydrolysis of p-nitrostyrene oxide (pNSO). In this study, the...
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Article
scFv antibodies against infectious bursal disease virus isolated from a combinatorial antibody library by flow cytometry
Infectious bursal disease is an economically important disease that affects chickens worldwide. Here, a recombinant single chain variable fragment (scFv) antibody library derived from chickens immunized with V...