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  1. Article

    Open Access

    Natural resistance to Potato virus Y in Solanum tuberosum Group Phureja

    Novel major gene resistance against Potato virus Y in diploid populations of Solanum tuberosum Groups Phureja and Tuberosum was biologically and genetically characterised. Named Ry(o)phu, it mapped to chromosome ...

    Lesley Torrance, Graham H. Cowan, Karen McLean… in Theoretical and Applied Genetics (2020)

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    Article

    Crops that feed the world 8: Potato: are the trends of increased global production sustainable?

    Potato is produced on all continents except Antarctica and is the world’s third most important food crop. Potato production has increased dramatically in develo** countries in the past two decades, and has n...

    Paul R. J. Birch, Glenn Bryan, Brian Fenton, Eleanor M. Gilroy, Ingo Hein… in Food Security (2012)

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    Article

    In vivo expression and binding activity of scFv-RWAV, which recognizes the coat protein of tomato leaf curl New Delhi virus (family Geminiviridae)

    Recombinant antibodies expressed in plants have the potential to interrupt virus infections by blocking essential stages of the infection cycle. Here, we show that the expression of a recombinant single-chain ...

    Adel M. Zakri, Angelika Ziegler, Ulrich Commandeur, Rainer Fischer… in Archives of Virology (2012)

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    Article

    Plasmodesmata viewed as specialised membrane adhesion sites

    A significant amount of work has been expended to identify the elusive components of plasmodesmata (PD) to help understand their structure, as well as how proteins are targeted to them. This review focuses on ...

    Jens Tilsner, Khalid Amari, Lesley Torrance in Protoplasma (2011)

  5. No Access

    Article

    Generation and characterization of a scFv against recombinant coat protein of the geminivirus tomato leaf curl New Delhi virus

    We report the establishment of a hybridoma cell line secreting the monoclonal antibody (mAb) HAV, which recognizes the coat (AV1) protein of tomato leaf curl New Delhi virus (ToLCNDV), a begomovirus. The cell ...

    Adel M. Zakri, Angelika Ziegler, Lesley Torrance, Rainer Fischer… in Archives of Virology (2010)

  6. No Access

    Article

    Comparative sequence analysis and serological and infectivity studies indicate that cocksfoot mild mosaic virus is a member of the genus Panicovirus

    The complete nucleotide sequence of the Phleum isolate of cocksfoot mild mosaic virus (CMMV-P) and the coat protein sequence of the cocksfoot isolate (CMMV-1) were determined. Comparative sequence analysis reveal...

    Angelika Ziegler, Graham Cowan, Lesley Torrance in Archives of Virology (2009)

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    Protocol

    Studies of the Role and Function of Barley Stripe Mosaic Virus Encoded Proteins in Replication and Movement Using GFP Fusions

    This chapter describes techniques to investigate the localisation and function of virus-encoded proteins in plants using green fluorescent protein (GFP) transiently expressed from plasmids or infectious cDNA r...

    Sophie Haupt, Angelika Ziegler, Graham Cowan… in Viral Applications of Green Fluorescent Pr… (2009)

  8. No Access

    Protocol

    Localization of Viral Proteins in Plant Cells: Protein Tagging

    This chapter describes techniques for in vivo imaging of fluorescent fusion proteins in living cells by confocal laser scanning microscopy (CLSM). Methods are provided for (i) producing the constructs for tran...

    Sophie Haupt, Angelika Ziegler, Lesley Torrance in Plant Virology Protocols (2008)

  9. No Access

    Protocol

    Role of Plant Virus Movement Proteins

    Plant viruses spread from the initially infected cells to the rest of the plant in several distinct stages. First, the virus (in the form of virions or nucleic acid protein complexes) moves intracellularly fro...

    Michael Taliansky, Lesley Torrance, Natalia O. Kalinina in Plant Virology Protocols (2008)

  10. No Access

    Article

    Facile assessment of cDNA constructs for expression of functional antibodies in plants using the potato virus X vector

    Antibodies have been expressed in plants to confer novel traits such as virus resistance or altered phenotype. However, not every antibody is suitable for plant expression, and successful intracellular express...

    Angelika Ziegler, Graham H. Cowan, Lesley Torrance, Heather A. Ross… in Molecular Breeding (2000)

  11. No Access

    Article

    Recombinant antibody fragments that detect enoyl acyl carrier protein reductase in Brassica napus

    Purified Brassica napus enoyl acyl carrier protein reductase (ENR) was used to select specific antibodies from a library of antibody fragments, single-chain Fv (scFv), displayed on filamentous phage. Analysis of ...

    Angelika Ziegler, Sybil M. Macintosh, Lesley Torrance, William Simon… in Lipids (1997)

  12. No Access

    Article

    Use of monoclonal antibodies in plant pathology

    Lesley Torrance in European Journal of Plant Pathology (1995)

  13. No Access

    Article

    Improved efficiency of detection of potato mop-top furovirus in potato tubers and in the roots and leaves of soil-bait plants

    A reverse trancription-polymerase chain reaction (RT-PCR) assay was devised and shown to be sensitive and reliable for the detection of potato mop-top virus (PMTV) RNA sequences in the flesh of virus infected ...

    Mohammed Arif, Lesley Torrance, Brian Reavy in Potato Research (1994)